对氨基马尿酸转运的底物刺激：对摄取和流出的影响。

Substrate stimulation of para-aminohippuric acid transport: effect on uptake and runout.

作者信息

Pegg D G, Hewitt W R, Hook J B

出版信息

Proc Soc Exp Biol Med. 1975 Jun;149(2):546-9. doi: 10.3181/00379727-149-38847.

Abstract

The ability of penicillin pretreatment to increase PAH accumulation by slices of newborn rabbit renal cortex was dissected into two components, uptake and runout. The oxygen-requiring component of the uptake process was significantly enhanced by penicillin treatment, whereas runout was unaffected. Kinetically, the data suggest that penicillin alters the affinity of the transport system for PAH. Due to the limitations of such a kinetic analysis, no conclusions may be drawn from such a suggestion. However, it may be concluded that penicillin pretreatment increases renal accumulation of PAH solely by stimulating the uptake process. Elucidation of the molecular changes involved will require techniques more sophisticated than uptake into renal cortical slices.

摘要

青霉素预处理增加新生兔肾皮质切片对对氨基马尿酸（PAH）蓄积的能力被分解为两个部分，摄取和流出。青霉素处理显著增强了摄取过程中需氧的部分，而流出不受影响。从动力学角度来看，数据表明青霉素改变了转运系统对PAH的亲和力。由于这种动力学分析存在局限性，无法从这一推测得出结论。然而，可以得出结论，青霉素预处理仅通过刺激摄取过程来增加肾脏对PAH的蓄积。要阐明其中涉及的分子变化，需要比肾皮质切片摄取更为复杂的技术。