das Neves R P, Santos T M, de Pereira M L, de Jesus J P
Departamento de Biologia, Universidade de Aveiro, Portugal,
Cytobios. 2001;106 Suppl 1:27-34.
Cr(VI), the highest oxidation state for chromium, is a carcinogenic and mutagenic agent. In vivo and in vitro Cr(VI) toxic effects are related to its intracellular fate. Once inside the cell it is reduced to stable Cr(III) by cysteine, glutathione and ascorbic acid. Additionally, as Cr(V) and/or Cr(IV) intermediates have been reported in Cr(VI) reactions with biological reductants, chromium damage is thought to originate from these chemical species. This work investigated the morphology of splenic cells after short-term exposure to Cr(VI). A dose of 30 mg of K2CrO4/kg body weight was administered to mice and the effects were studied 24 and 48 h after the injections. Histological results revealed a time-dependency effect of Cr(VI) on splenic cells. Changes included enlargement of the capsule and depletion of the red pulp cells, accompanied by an increase in macrophages, 24 h after injection. Partial restoration of red pulp was noted after 48 h.
铬的最高氧化态六价铬是一种致癌和致突变剂。六价铬在体内和体外的毒性作用与其在细胞内的命运有关。一旦进入细胞，它会被半胱氨酸、谷胱甘肽和抗坏血酸还原为稳定的三价铬。此外，由于在六价铬与生物还原剂的反应中已报道有五价铬和/或四价铬中间体，所以认为铬的损伤源于这些化学物质。这项工作研究了短期暴露于六价铬后脾细胞的形态。给小鼠注射30毫克/千克体重的重铬酸钾，并在注射后24小时和48小时研究其影响。组织学结果显示六价铬对脾细胞具有时间依赖性效应。变化包括注射后24小时包膜增厚和红髓细胞减少，同时巨噬细胞增多。48小时后红髓有部分恢复。
