Nakamura T J, Shinohara K, Funabashi T, Mitsushima D, Kimura F
Department of Physiology, Yokohama City University School of Medicine, 236-0004, Yokohama, Japan.
Neurosci Res. 2001 Sep;41(1):25-32. doi: 10.1016/s0168-0102(01)00255-3.
Expressions of Cry1 and Cry2 mRNA in the rat pineal gland were examined by Northern blot. The levels of Cry1 and Cry2 transcript had a marked circadian rhythm with peaks at circadian time (CT) 20 in constant darkness. But the amplitude of the Cry1 rhythm was higher than that of the Cry2 rhythm. Furthermore, a significant increase in Cry1 mRNA levels was caused by light pulse given at CT 16 but not at CT 4, but the expression of Cry2 was not significantly induced by light pulses given at either CT 4 or CT 16. These results suggest that Cry1 in the pineal gland is regulated by photic and circadian information but Cry2 is only regulated by circadian information.
通过Northern印迹法检测大鼠松果体中Cry1和Cry2 mRNA的表达。在持续黑暗条件下，Cry1和Cry2转录本水平呈现明显的昼夜节律，在昼夜时间（CT）20时达到峰值。但Cry1节律的幅度高于Cry2节律。此外，在CT 16给予光脉冲可导致Cry1 mRNA水平显著升高，而在CT 4给予光脉冲则无此效果，并且在CT 4或CT 16给予光脉冲均未显著诱导Cry2的表达。这些结果表明，松果体中的Cry1受光信号和昼夜节律信息的调节，而Cry2仅受昼夜节律信息的调节。
