Nayci A, Cakmak M, Aksoyek S, Renda N, Yucesan S
Department of Pediatric Surgery, Mersin University Medical Faculty, PK 33070, Mersin, Turkey.
Dis Colon Rectum. 2001 Aug;44(8):1181-8. doi: 10.1007/BF02234642.
Magnetic fields have been shown to affect biologic processes. Accordingly, an experimental study was designed to investigate the effect of electromagnetic field stimulation on intestinal healing and to compare small and large intestinal anastomoses.
An ileal or a colonic anastomosis was constructed in rats. Beginning the day after surgery, randomly assigned groups were exposed to sinusoidal electromagnetic field stimulation of 10.76-mT intensity and 50-Hz frequency, with 2-hour-on/10-hour-off cycles. After seven days, intestinal anastomoses were assessed for hydroxyproline content and breaking strength. Statistical comparison between each experimental and control group yielded significance (P < 0.05) in all cases.
Hydroxyproline content increased significantly in ileum from 1.650 +/- 0.11 (mean +/- standard error of the mean) to 2.036 +/- 0.11 microg/mg (P = 0.0249) and in colon from 1.526 +/- 0.11 to 1.922 +/- 0.11 microg/mg (P = 0.0135). Breaking strength also increased significantly in ileum from 0.213 +/- 0.01 to 0.255 +/- 0.01 MPa (P = 0.001) and in colon from 0.227 +/- 0.01 to 0.270 +/- 0.01 MPa (P = 0.006).
Electromagnetic field stimulation provided a significant gain in anastomotic healing in both small and large intestine. There were no apparent differences detected between the healing of small and large intestinal anastomoses except for slight differences in the time sequences of events and magnitude. The study demonstrated a significant increase in both biochemical and mechanical parameters. Additional investigations are needed to determine optimal conditions and promote selective biologic responses.
磁场已被证明会影响生物过程。因此，设计了一项实验研究来调查电磁场刺激对肠道愈合的影响，并比较小肠和大肠吻合术。
在大鼠身上构建回肠或结肠吻合术。从手术后第一天开始，随机分组的大鼠暴露于强度为10.76 mT、频率为50 Hz的正弦电磁场刺激下，刺激周期为2小时开启/10小时关闭。七天后，评估肠道吻合处的羟脯氨酸含量和断裂强度。各实验组与对照组之间的统计学比较在所有情况下均具有显著性（P < 0.05）。
回肠中的羟脯氨酸含量从1.650±0.11（平均值±平均标准误差）显著增加至2.036±0.11微克/毫克（P = 0.0249），结肠中的羟脯氨酸含量从1.526±0.11增加至1.922±0.11微克/毫克（P = 0.0135）。回肠的断裂强度也从0.213±0.01显著增加至0.255±0.01兆帕（P = 0.001），结肠的断裂强度从0.227±0.01增加至0.270±0.01兆帕（P = 0.006）。
电磁场刺激在小肠和大肠的吻合口愈合方面均有显著改善。除了在事件发生的时间顺序和程度上存在细微差异外，小肠和大肠吻合口的愈合情况未发现明显差异。该研究表明生化和力学参数均有显著增加。需要进一步研究以确定最佳条件并促进选择性生物反应。
