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一种用于鉴定淋病奈瑟菌的培养方法的评估

Evaluation of a culture procedure for identification of Neisseria gonorrhoeae.

作者信息

Hellgren L, Johannison G, Vincent J

出版信息

Ann Clin Res. 1979 Jun;11(3):98-100.

PMID:115369
Abstract

A new rapid culture technique (Microcult GC) for identification of Neisseria gonorrhoeae was compared with a standard culture procedure in 151 patients (59 males, 92 females) suffering from urethritis and/or cervicitis. In 86.8% of the patients identical results were achieved when the new and the standard culture techniques were compared. In 4% the new culture technique revealed N. gonorrhoeae, confirmed by Bram-stain microscopy, when the standard culture was negative. However in 9.1% the reverse was true. In conclusion the new technique showed a decrease of 5% compared with the standard culture method. None of the methods gave rise to detectable false reactions. The new culture procedure might be a complement in the diagnosis of gonorrhoea because of its ease and rapidity (results are available after 24 h) but the method should not be used as a single diagnostic as it suffers from an unacceptable non-specifity especially in women.

摘要

一种用于鉴定淋病奈瑟菌的新型快速培养技术(微需氧GC培养法)与标准培养程序在151例患有尿道炎和/或宫颈炎的患者(59名男性,92名女性)中进行了比较。当比较新的培养技术和标准培养技术时,86.8%的患者获得了相同的结果。在4%的患者中,当标准培养为阴性时,新的培养技术检测出淋病奈瑟菌,经革兰氏染色显微镜检查证实。然而在9.1%的患者中情况相反。总之,与标准培养方法相比,新技术的检出率降低了5%。两种方法均未产生可检测到的假反应。由于其简便性和快速性(24小时后即可获得结果),新的培养程序可能是淋病诊断中的一种补充方法,但该方法不应作为单一诊断方法使用,因为它存在不可接受的非特异性,尤其是在女性中。

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