重离子对细菌的诱变效应。

Mutagenic effects of heavy ions in bacteria.

作者信息

Krasavin E A, Kozubek S, Amirtayev K G, Tokarova B, Bonev M

机构信息

Joint Institute for Nuclear Research, Dubna, USSR.

出版信息

Adv Space Res. 1992;12(2-3):103-10. doi: 10.1016/0273-1177(92)90096-g.

DOI:10.1016/0273-1177(92)90096-g

PMID:11536997

Abstract

The peculiarities and mechanisms of the mutagenic action of gamma-rays and heavy ions on bacterial cells have been investigated. Direct mutations in the lac-operon of E. coli in wild type cells and repair deficient strains have been detected. Furthermore, the induction of revertants in Salmonella tester strains was measured. It was found that the mutation rate was a linear-quadratic function of dose in the case of both gamma-rays and heavy ions with LET up to 200 keV/micrometer. The relative biological effectiveness (RBE) increased with LET up to 20 keV/micrometer. Low mutation rates were observed in repair deficient mutants with a block of SOS-induction. The induction of SOS-repair by ionizing radiation has been investigated by means of the "SOS-chromotest" and lambda-prophage induction. It was shown that the intensity of the SOS-induction in E. coli increased with increasing LET up to 40-60 keV/micrometer.

摘要

已经研究了γ射线和重离子对细菌细胞诱变作用的特点和机制。在野生型细胞和修复缺陷型菌株的大肠杆菌乳糖操纵子中检测到了直接突变。此外，还测量了沙门氏菌测试菌株中回复突变体的诱导情况。结果发现，对于γ射线和线性能量传递（LET）高达200 keV/微米的重离子，突变率是剂量的线性二次函数。相对生物效能（RBE）随着LET增加到20 keV/微米而增加。在具有SOS诱导阻断的修复缺陷型突变体中观察到低突变率。通过“SOS色测试”和λ噬菌体诱导研究了电离辐射对SOS修复的诱导作用。结果表明，大肠杆菌中SOS诱导的强度随着LET增加到40 - 60 keV/微米而增加。