Hicks G R, Rayle D L, Lomax T L
Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331-2902.
Science. 1989 Jul 7;245:52-4. doi: 10.1126/science.245.4913.52.
Tomato plants homozygous for the diageotropica (dgt) mutation exhibit morphological and physiological abnormalities which suggest that they are unable to respond to the plant growth hormone auxin (indole-3-acetic acid). The photoaffinity auxin analog [3H]5N3-IAA specifically labels a polypeptide doublet of 40 and 42 kilodaltons in membrane preparations from stems of the parental variety, VFN8, but not from stems of plants containing the dgt mutation. In roots of the mutant plants, however, labeling is indistinguishable from that in VFN8. These data suggest that the two polypeptides are part of a physiologically important auxin receptor system, which is altered in a tissue-specific manner in the mutant.
对于双生突变（dgt）纯合的番茄植株表现出形态和生理异常，这表明它们无法对植物生长激素生长素（吲哚 - 3 - 乙酸）作出反应。光亲和生长素类似物[3H]5N3 - IAA在亲本品种VFN8茎的膜制剂中特异性标记了40和42千道尔顿的多肽双峰，但在含有dgt突变的植株茎中则没有。然而，在突变植株的根中，标记与VFN8中的标记没有区别。这些数据表明，这两种多肽是生理上重要的生长素受体系统的一部分，该系统在突变体中以组织特异性方式发生了改变。
