Neff A W, Smith R C, Malacinski G M
Medical Sciences Program, School of Medicine, Indiana University, Bloomington 47405.
Adv Space Res. 1986;6(12):21-8. doi: 10.1016/0273-1177(86)90061-x.
Elucidation of dorsal/ventral polarity and primary embryonic axis development in amphibian embryos requires an understanding of cytoplasmic rearrangements in fertile eggs at the biophysical, physiological, and biochemical levels. Evidence is presented that amphibian egg cytoplasmic components are compartmentalized. The effects of altered orientation to the gravitational vector (i.e., egg inversion) and alterations in gravity force ranging from hypergravity (centrifugation) to simulated microgravity (i.e., horizontal clinostat rotation) on cytoplasmic compartment rearrangements are reviewed. The behavior of yolk compartments as well as a newly defined (with monoclonal antibody) non-yolk cytoplasmic compartment, in inverted eggs and in eggs rotated on horizontal clinostats at their buoyant density, is discussed.
阐明两栖类胚胎的背/腹极性和原胚轴发育需要在生物物理、生理和生化水平上了解可育卵中的细胞质重排。有证据表明两栖类卵的细胞质成分是分隔的。本文综述了重力矢量方向改变（即卵倒置）以及从超重力（离心）到模拟微重力（即水平回转器旋转）的重力变化对细胞质分隔重排的影响。讨论了卵黄区室以及一个新定义的（用单克隆抗体）非卵黄细胞质区室在倒置卵和在水平回转器上以其浮力密度旋转的卵中的行为。
