Claassen D E, van Twest J S, Spooner B S
Division of Biology, NSCORT, Kansas State University, Manhattan 66506.
Adv Space Res. 1994;14(8):111-4. doi: 10.1016/0273-1177(94)90393-x.
Shuttle flight, sounding rocket flight, and parabolic flight experiments demonstrate the formation of bilayer membrane vesicles (liposomes) in reduced gravity, following the dilution of detergent from detergent-phospholipid mixed micelles. The reduction in detergent concentration initiates assembly of bilayer membrane sheets, which are sensitive to solution disturbances. An increase in disturbances by forced dilution results in small diameter liposomes (< 150 nm), in both ground and flight samples. In the absence of forced dilution, liposomes remain small at 1-g, but exhibit much larger diameters at 0-g (1000-2000 nm). Our spaceflight data reveal that membrane assembly and vesiculation are strongly influenced by gravity-induced solution disturbances (e.g., convection currents), which limit vesicle diameter.
航天飞机飞行、探空火箭飞行和抛物线飞行实验表明，在将洗涤剂从洗涤剂 - 磷脂混合胶束中稀释后，双层膜囊泡（脂质体）在微重力环境下形成。洗涤剂浓度的降低引发双层膜片的组装，这些膜片对溶液扰动敏感。在地面和飞行样本中，通过强制稀释增加扰动会导致小直径脂质体（<150 nm）的形成。在没有强制稀释的情况下，脂质体在1g重力下保持较小，但在0g重力下呈现更大的直径（1000 - 2000 nm）。我们的航天飞行数据表明，膜组装和囊泡化受到重力引起的溶液扰动（如对流）的强烈影响，这些扰动限制了囊泡直径。
