Frick J, Mitchell C A
Department of Horticulture, Purdue University, West Lafayette, IN 47907-1165, USA.
HortScience. 1993 Oct;28(10):981-4.
2-[N-morpholino]ethanesulfonic acid (MES) buffer or Amberlite DP-1 (cation-exchange resin beads) were used to stabilize substrate pH of passive-wicking, solid-matrix hydroponic systems in which small canopies of Brassica napus L. (CrGC 5-2, genome : ACaacc) were grown to maturity. Two concentrations of MES (5 or 10 mM) were included in Hoagland 1 nutrient solution. Alternatively, resin beads were incorporated into the 2 vermiculite : 1 perlite (v/v) growth medium at 6% or 12% of total substrate volume. Both strategies stabilized pH without toxic side effects on plants. Average seed yield rates for all four pH stabilization treatments (13.3 to 16.9 g m-2 day-1) were about double that of the control (8.2 g m-2 day-1), for which there was no attempt to buffer substrate pH. Both the highest canopy seed yield rate (16.9 g m-2 day-1) and the highest shoot harvest index (19.5%) occurred with the 6% resin bead treatment, even though the 10 mM MES and 12% bead treatments maintained pH within the narrowest limits. The pH stabilization methods tested did not significantly affect seed oil and protein contents.
2-[N-吗啉代]乙磺酸（MES）缓冲液或Amberlite DP-1（阳离子交换树脂珠）用于稳定被动吸水固体基质水培系统的基质pH值，在该系统中，甘蓝型油菜（CrGC 5-2，基因组：ACaacc）的小植株生长至成熟。霍格兰1号营养液中包含两种浓度的MES（5或10 mM）。或者，将树脂珠以占总基质体积6%或12%的比例掺入2份蛭石:1份珍珠岩（v/v）的生长培养基中。两种策略都能稳定pH值，且对植物没有毒性副作用。所有四种pH稳定处理的平均种子产量率（13.3至16.9克/平方米·天）约为对照（8.2克/平方米·天）的两倍，对照未尝试缓冲基质pH值。即使10 mM MES和12%树脂珠处理能将pH维持在最窄范围内，但最高的冠层种子产量率（16.9克/平方米·天）和最高的地上部收获指数（19.5%）都出现在6%树脂珠处理中。所测试的pH稳定方法对种子油和蛋白质含量没有显著影响。
