Frazer L N, Moore D
School of Biological Sciences, The University of Manchester, U.K.
Adv Space Res. 1996;17(6-7):87-90. doi: 10.1016/0273-1177(95)00616-m.
The role of Ca2+ in the gravitropic perception and/or response mechanism of Coprinus cinereus was examined by treating stipes with inhibitors of Ca2+ transport and calmodulin. Inhibitors had no effect on gravity perception but significantly diminished gravitropism. It is concluded that, under the conditions tested, Ca2+ is not involved in gravity perception by Coprinus stipes, but does contribute to transduction of the gravitropic impulse. The results would be consistent with regulation of the gravitropic bending process requiring accumulation of Ca2+ within a membrane-bound compartment. Treatment of stipes with an actin inhibitor caused a significantly delayed response, a result not observed with the Ca2+ inhibitors. This suggests that cytoskeletal elements may be involved directly in perception of gravity by Coprinus stipes while Ca(2+)-mediated signal transduction may be involved in directing growth differentials.
通过用钙离子运输抑制剂和钙调蛋白处理鸡腿蘑菌柄，研究了钙离子在鸡腿蘑重力感知和/或反应机制中的作用。抑制剂对重力感知没有影响，但显著减弱了向地性。得出的结论是，在测试条件下，钙离子不参与鸡腿蘑菌柄的重力感知，但确实有助于重力感应冲动的传导。该结果与重力弯曲过程的调节需要钙离子在膜结合区室中积累相一致。用肌动蛋白抑制剂处理菌柄会导致反应显著延迟，而钙离子抑制剂处理则未观察到这一结果。这表明细胞骨架成分可能直接参与鸡腿蘑菌柄的重力感知，而钙离子介导的信号转导可能参与引导生长差异。
