Popova A F, Sytnik K M
Institute of Botany, National Academy Sciences of Ukraine, Kiev.
Adv Space Res. 1996;17(6-7):99-102. doi: 10.1016/0273-1177(95)00619-p.
The ultrastructure of Chlorella cells grown in darkness on a solid agar medium with organic additions aboard the Bion-10 biosatellite was studied. Certain differences in submicroscopic organization of organelles in the experimental cells were revealed compared to the Earth control. The changes are registered mainly in ultrastructure of energetic organelles--mitochondria and plastids of the experimental cells, in particular, an increase of mitochondria and their cristae size, as well as an increase of the total volume of mitochondrion per cell were established. The decrease of the starch amount in the plastid stroma and the electron density of the latter was also observed. In many experimental cells, the increase of condensed chromatin in the nuclei has been noted. Ultrastructural rearrangements in cells after laboratory experiment realized according to the thermogram registered aboard the Bion-10 were insignificant compared to the flight experiment. Data obtained are compared to results of space flight experiments carried out aboard the Bion-9 (polycomponent aquatic system) and the orbital station Mir (solid agar medium).
对搭载“生物-10号”生物卫星在黑暗环境下于添加了有机物的固体琼脂培养基上生长的小球藻细胞的超微结构进行了研究。与地面对照组相比,实验细胞的细胞器亚显微结构存在某些差异。这些变化主要记录在实验细胞的能量细胞器——线粒体和质体的超微结构中,具体而言,发现线粒体数量增加、其嵴的尺寸增大,以及每个细胞中线粒体的总体积增加。还观察到质体基质中淀粉量减少及其电子密度降低。在许多实验细胞中,已注意到细胞核中凝聚染色质增加。与飞行实验相比,根据“生物-10号”上记录的热成像图在实验室进行实验后细胞的超微结构重排不明显。将所获得的数据与在“生物-9号”(多组分水生系统)和和平号轨道站上进行的太空飞行实验(固体琼脂培养基)的结果进行了比较。