Gopinath C, Howell J M
Res Vet Sci. 1975 Jul;19(1):35-43.
Eight sheep were given daily oral doses of copper sulphate until haemolysis occurred. Three of the sheep developed further periods of haemolysis after dosing ceased. Serum enzyme and urea levels were measured throughout the experiment and compared to those obtained from three undosed control sheep. Serum enzyme levels rose prior to haemolytic crises and urea levels rose subsequent to haemolysis in animals that died or were killed in extremis. Severe morphological changes were seen in liver, kidney and brain. Tissue levels of copper and iron were markedly elevated. It is concluded that tissue damage continues even after the cessation of ingestion of copper and that the damage can be severe enough to lead to repeated haemolytic crises.
给八只绵羊每日口服硫酸铜直至发生溶血。其中三只绵羊在停止给药后又出现了溶血期。在整个实验过程中测量血清酶和尿素水平，并与三只未给药的对照绵羊的测量结果进行比较。在发生溶血危机之前血清酶水平升高，在濒死或极度危急状态下处死的动物中，溶血后尿素水平升高。在肝脏、肾脏和大脑中观察到严重的形态学变化。铜和铁的组织水平显著升高。得出的结论是，即使在停止摄入铜之后，组织损伤仍会持续，并且这种损伤可能严重到足以导致反复的溶血危机。
