5'-AMP的N-乙酰-D-酯和L-酯以不同速率水解。

N-Acetyl-D- and L-esters of 5'-AMP hydrolyze at different rates.

作者信息

Wickramasinghe N S, Lacey J C

机构信息

Department of Biochemistry, University of Alabama at Birmingham, USA.

出版信息

Chirality. 1993;5:150-3. doi: 10.1002/chir.530050308.

DOI:10.1002/chir.530050308

PMID:11540505

Abstract

Studies of the properties of aminoacyl derivatives of 5'-AMP are aimed at understanding the origin of the process of protein synthesis. Aminoacyl (2',3') esters of 5'-AMP can serve as models of the 3'-terminus of aminoacyl tRNA. We report here on the relative rates of hydrolysis of Ac-D- and L-Phe AMP esters as a function of pH. At all pHs above 3, the rate constant of hydrolysis of the Ac-L-Phe ester is 1.7 to 2.1 times that of Ac-D-Phe ester. The D-isomer seems partially protected from hydrolysis by a stronger association with the adenine ring of the 5'-AMP.

摘要

对5'-AMP氨酰基衍生物性质的研究旨在了解蛋白质合成过程的起源。5'-AMP的氨酰基（2',3'）酯可作为氨酰基tRNA 3'-末端的模型。我们在此报告了Ac-D-和L-苯丙氨酸AMP酯水解的相对速率与pH的关系。在pH高于3的所有情况下，Ac-L-苯丙氨酸酯的水解速率常数是Ac-D-苯丙氨酸酯的1.7至2.1倍。D-异构体似乎通过与5'-AMP的腺嘌呤环更强的缔合而部分免受水解。