Batkai L, Varkonyi A, Minarovits J
National Center for Epidemiology, Microbiological Research Group, Budapest.
J Gravit Physiol. 1999 Jul;6(1):P109-10.
Our earlier space experiments demonstrated that the interferon production of human lymphocytes in microgravity is 4-8 times higher than those of the synchronous ground controls in vitro (Talas et al. 1983). These data suggested that the microgravity has a significant effect on cells. Since the possibilities to perform space-experiments are very limited and our study raised many interesting questions, we wished to simulate microgravity conditions in our laboratory. For this reason we purchased a Rotary Cell Culture System (RCCS) equipment to study different cell lines and human peripheral blood mononuclear cells (PBMCs) in experimental microgravity conditions. RCCS is a horizontally rotated bubble free culture vessel with membrane diffusion gas exchange. We report here an analysis of TNF-alpha (tumor necrosis factor-alpha) production by human PBMCs (control cultures exposed to simulated microgravity in RCCS). The cells were incubated in the presence or absence of either NDV (Newcastle Disease Virus) or one of the different forms (PHA-M or -P) of Phytohaemagglutinin.
我们早期的太空实验表明，在微重力条件下，人淋巴细胞产生干扰素的量比同步地面对照体外实验高出4至8倍（塔拉斯等人，1983年）。这些数据表明，微重力对细胞有显著影响。由于进行太空实验的可能性非常有限，且我们的研究提出了许多有趣的问题，我们希望在实验室模拟微重力条件。因此，我们购买了旋转细胞培养系统（RCCS）设备，以研究不同细胞系和人外周血单个核细胞（PBMC）在实验性微重力条件下的情况。RCCS是一个水平旋转的无泡培养容器，具有膜扩散气体交换功能。我们在此报告对人PBMC（在RCCS中暴露于模拟微重力的对照培养物）产生肿瘤坏死因子-α（TNF-α）的分析。细胞在有或没有新城疫病毒（NDV）或不同形式（PHA-M或-P）的植物血凝素存在的情况下进行培养。
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