Sanford G L, Harris-Hooker S, Lui J, Bosah F N
Space Medicine & Life Sciences Research Center, Morehouse School of Medicine, Atlanta, Georgia, USA.
J Gravit Physiol. 1999 Jul;6(1):P29-30.
Anticipated hazards for crewmembers in future long-term space flights may result in a variety of injuries including fractures, deep punctures or cuts. The microgravity environment of space may complicate the wound healing process. Myofibroblasts have been proposed to play a role in wound contraction; these cells develop from tissue fibroblasts sue fibroblasts develop numerous features found in vascular smooth muscle cells (SMC), ultrastructural features, expression of alpha-SM actin and microfilament bundles. These changes have been shown to be inducible by TGF beta 1. Previous studies have also shown that TGF beta 1 is capable of initiating and regulating critical events in bone fracture, soft tissue, dermal wound healing. Several studies have suggested that bFGF may also be involved in the wound healing process, and that the interactions of bFGF with TGF beta 1 control the overall repair of a wounded tissue. The formation (angiogenesis) and/or repair of blood vessels is also essential for wound healing. Both TGF beta 1 and bFGF have been shown to affect both angiogenesis and vascular injury repair. However, the response of cells following injury, in a microgravity or hypergravity (HG) environment has not been evaluated. We assessed the influence of HG (centrifugation at 6G) and clinostat rotation at 30 rpm (CR) on the response of SMC to a denudation injury. We also examined the possible involvement of c-myc, c-fos and TGF beta 1 in meeting the response of SMC to wounding.
未来长期太空飞行中机组人员可能面临的危害可能导致包括骨折、深部刺伤或割伤在内的各种损伤。太空的微重力环境可能会使伤口愈合过程变得复杂。有人提出肌成纤维细胞在伤口收缩中起作用；这些细胞由组织成纤维细胞发育而来，成纤维细胞具有许多在血管平滑肌细胞（SMC）中发现的特征、超微结构特征、α-SM肌动蛋白的表达和微丝束。这些变化已被证明可由转化生长因子β1诱导。先前的研究还表明，转化生长因子β1能够启动和调节骨折、软组织、皮肤伤口愈合中的关键事件。几项研究表明，碱性成纤维细胞生长因子（bFGF）也可能参与伤口愈合过程，并且bFGF与转化生长因子β1的相互作用控制受伤组织的整体修复。血管的形成（血管生成）和/或修复对于伤口愈合也至关重要。转化生长因子β1和bFGF都已被证明会影响血管生成和血管损伤修复。然而，在微重力或超重力（HG）环境下细胞受伤后的反应尚未得到评估。我们评估了超重力（6G离心）和30 rpm的回转器旋转（CR）对SMC对剥脱性损伤反应的影响。我们还研究了c-myc、c-fos和转化生长因子β1在满足SMC对损伤反应中的可能作用。
