己糖醇核酸模板上RNA和DNA寡聚物的非酶促合成：A结构的重要性。

Nonenzymatic synthesis of RNA and DNA oligomers on hexitol nucleic acid templates: the importance of the A structure.

作者信息

Kozlov I A, Politis P K, Van Aerschot A, Busson R, Herdewijn P, Orgel L E

机构信息

Salk Institute for Biological Studies, San Diego, California 92186, USA.

出版信息

J Am Chem Soc. 1999 Mar 31;121(12):2653-6. doi: 10.1021/ja983958r.

DOI:10.1021/ja983958r

PMID:11543583

Abstract

Hexitol nucleic acid (HNA) is an analogue of DNA containing the standard nucleoside bases, but with a phosphorylated 1,5-anhydrohexitol backbone. HNA oligomers form duplexes having the nucleic acid A structure with complementary DNA or RNA oligomers. The HNA decacytidylate oligomer is an efficient template for the oligomerization of the 5'-phosphoroimidazolides of guanosine or deoxyguanosine. Comparison of the oligomerization efficiencies on HNA, RNA, and DNA decacytidylate templates under various conditions suggests strongly that only nucleic acid double helices with the A structure support efficient template-directed synthesis when 5'-phosphoroimidazolides of nucleosides are used as substrates.

摘要

己糖醇核酸（HNA）是一种DNA类似物，含有标准核苷碱基，但具有磷酸化的1,5-脱水己糖醇主链。HNA寡聚物与互补的DNA或RNA寡聚物形成具有核酸A结构的双链体。HNA十聚胞苷酸寡聚物是鸟苷或脱氧鸟苷的5'-磷酰咪唑化物寡聚化的有效模板。在各种条件下对HNA、RNA和DNA十聚胞苷酸模板上的寡聚化效率进行比较，结果有力地表明，当使用核苷的5'-磷酰咪唑化物作为底物时，只有具有A结构的核酸双螺旋支持有效的模板导向合成。