Pretti C, Salvetti A, Longo V, Giorgi M, Gervasi P G
Dipartimento di Patologia Animale, Profilassi ed Igiene degli Alimenti, Università di Pisa, V.le delle Piagge 2, 56124, Pisa, Italy.
Comp Biochem Physiol C Toxicol Pharmacol. 2001 Sep;130(1):133-44. doi: 10.1016/s1532-0456(01)00231-9.
The effect of beta-naphthoflavone (beta-NF) on several catalytic activities of cytochrome P450 (CYP) and phase II enzymes putatively controlled by [Ah]-receptor activation in the liver, heart and kidney of gilthead seabream, was investigated. In the liver, beta-NF treatment [intraperitoneal injection (i.p.) 50 mg/kg] resulted in an increase of CYP content, immunoreactive CYP 1A and methoxyresorufin-O-demethylase (MEROD), pentoxyresorufin O-depentylase (PROD) and ethoxyresorufin-O-deethylase (EROD) activities. However, beta-NF had no effect on any of the hepatic phase II enzymes examined (benzaldehyde dehydrogenase, propionaldehyde dehydrogenase, glutathione S-transferase, UDP-glucuronyl-transferase, DT-diaphorase). Single i.p. injection of 10 mg/kg beta-NF showed a maximal induction of CYP 1A-like protein and EROD activity after 3-7 days. CYP 1A and EROD returned to control levels 18-days post-treatment. beta-NF injection also caused a rapid increase of a single band size of mRNA recognized by a CYP 1A1 cDNA fragment from sea bass (Dicentrarchus labrax). Expression of mRNA preceded the increase of EROD activity and declined rapidly by 96 h. Dose-response experiments demonstrated that EROD was significantly enhanced in liver by a single injection of 0.3 mg/kg beta-NF and was the most sensitive measurement for CYP 1A-like induction. beta-NF treatments also increased the expression of CYP 1A-like protein, mRNA and EROD, but not MEROD and PROD activities in heart and kidney.
研究了β-萘黄酮（β-NF）对金头鲷肝脏、心脏和肾脏中细胞色素P450（CYP）的几种催化活性以及推测由[Ah]受体激活控制的II相酶的影响。在肝脏中，β-NF处理[腹腔注射（i.p.）50mg/kg]导致CYP含量、免疫反应性CYP 1A以及甲氧基试卤灵-O-脱甲基酶（MEROD）、戊氧基试卤灵O-脱戊基酶（PROD）和乙氧基试卤灵-O-脱乙基酶（EROD）活性增加。然而，β-NF对所检测的任何肝脏II相酶（苯甲醛脱氢酶、丙醛脱氢酶、谷胱甘肽S-转移酶、UDP-葡萄糖醛酸基转移酶、DT-黄递酶）均无影响。单次腹腔注射10mg/kgβ-NF后，3-7天CYP 1A样蛋白和EROD活性出现最大诱导。处理后18天，CYP 1A和EROD恢复到对照水平。β-NF注射还导致鲈鱼（欧洲鲈）CYP 1A1 cDNA片段识别的单一条带大小的mRNA迅速增加。mRNA的表达先于EROD活性的增加，并在96小时内迅速下降。剂量反应实验表明，单次注射0.3mg/kgβ-NF可显著增强肝脏中的EROD，并且是CYP 1A样诱导的最敏感指标。β-NF处理还增加了心脏和肾脏中CYP 1A样蛋白、mRNA和EROD的表达，但未增加MEROD和PROD活性。
