肠杆菌科各属中rrl基因的间隔序列与23S rRNA的片段化
Intervening sequences in rrl genes and fragmentation of 23S rRNA in genera of the family Enterobacteriaceae.
作者信息
Pronk L M, Sanderson K E
机构信息
Salmonella Genetic Stock Centre, Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada T2N 1N4.
出版信息
J Bacteriol. 2001 Oct;183(19):5782-7. doi: 10.1128/JB.183.19.5782-5787.2001.
Abstract
Intervening sequences (IVSs) in the rrl genes for 23S rRNA are transcribed but later removed by RNase III without religation during RNA processing, leading to fragmented rRNA. We examined about 240 strains of the family Enterobacteriaceae for presence of IVSs using PCR. No IVSs were detected in strains belonging to Escherichia, Shigella, Enterobacter, Erwinia, Ewingella, Hafnia, Kluyvera, Morganella, Pantoea, or Serratia. Previously unreported IVSs were detected in Klebsiella oxytoca, Citrobacter amalonaticus, and Providencia stuartii; previously reported IVSs are in species of Salmonella, Proteus, Providencia, and Yersinia. The sporadic distribution of IVSs indicates lateral genetic transfer of IVSs.
摘要
23S rRNA的rrl基因中的间隔序列（IVSs）会被转录，但在RNA加工过程中随后会被RNase III去除且不会重新连接，从而导致rRNA片段化。我们使用PCR检测了约240株肠杆菌科细菌中IVSs的存在情况。在属于大肠杆菌、志贺氏菌、肠杆菌、欧文氏菌、尤因氏菌、哈夫尼亚菌、克吕韦氏菌、摩根氏菌、泛菌或沙雷氏菌的菌株中未检测到IVSs。在产酸克雷伯菌、无丙二酸柠檬酸杆菌和斯氏普罗威登斯菌中检测到了先前未报道的IVSs；先前报道的IVSs存在于沙门氏菌、变形杆菌、普罗威登斯菌和耶尔森氏菌的物种中。IVSs的零星分布表明IVSs存在横向基因转移。
相似文献
1
Intervening sequences in rrl genes and fragmentation of 23S rRNA in genera of the family Enterobacteriaceae.肠杆菌科各属中rrl基因的间隔序列与23S rRNA的片段化
J Bacteriol. 2001 Oct;183(19):5782-7. doi: 10.1128/JB.183.19.5782-5787.2001.
2
Fragmentation of 23S rRNA in strains of Proteus and Providencia results from intervening sequences in the rrn (rRNA) genes.变形杆菌属和普罗威登斯菌属菌株中23S rRNA的片段化是由rrn（rRNA）基因中的间隔序列导致的。
J Bacteriol. 2000 Feb;182(4):1109-17. doi: 10.1128/JB.182.4.1109-1117.2000.
3
Sequence diversity of intervening sequences (IVSs) in the 23S ribosomal RNA in Salmonella spp.沙门氏菌属23S核糖体RNA中插入序列（IVSs）的序列多样性
Gene. 2000 Jul 25;253(1):55-66. doi: 10.1016/s0378-1119(00)00239-0.
4
Distribution of intervening sequences in the genes for 23S rRNA and rRNA fragmentation among strains of the Salmonella reference collection B (SARB) and SARC sets.沙门氏菌参考菌株集B（SARB）和SARC组中23S rRNA基因间序列的分布及rRNA片段化情况。
J Bacteriol. 2000 Apr;182(7):1923-9. doi: 10.1128/JB.182.7.1923-1929.2000.
5
Intervening sequences in 23S rRNA genes and 23S rRNA fragmentation in Taylorella asinigenitalis UCD-1(T) strain.马源泰勒氏菌UCD-1(T)菌株23S rRNA基因中的间隔序列和23S rRNA片段化
J Basic Microbiol. 2008 Aug;48(4):284-92. doi: 10.1002/jobm.200700386.
6
Salmonella typhimurium LT2 possesses three distinct 23S rRNA intervening sequences.鼠伤寒沙门氏菌LT2拥有三个不同的23S核糖体RNA间隔序列。
J Bacteriol. 1996 Apr;178(8):2272-8. doi: 10.1128/jb.178.8.2272-2278.1996.
7
Demonstration of the absence of intervening sequences within 23S rRNA genes from Campylobacter lari.弯曲杆菌23S rRNA基因中不存在间隔序列的证明。
J Basic Microbiol. 2009 Aug;49(4):386-94. doi: 10.1002/jobm.200800232.
8
Identification and characterization of intervening sequences within 23S rRNA genes from more than 200 Campylobacter isolates from seven species including atypical campylobacters.鉴定和分析来自 7 个种（包括非典型弯曲菌）的 230 多个弯曲杆菌分离株 23S rRNA 基因内的插入序列。
BMC Microbiol. 2009 Dec 11;9:256. doi: 10.1186/1471-2180-9-256.
9
Identification and characterization of an intervening sequence within the 23S ribosomal RNA genes of Edwardsiella ictaluri.斑点叉尾鮰爱德华氏菌23S核糖体RNA基因中一个间隔序列的鉴定与表征
Syst Appl Microbiol. 2007 Mar;30(2):93-101. doi: 10.1016/j.syapm.2006.04.004. Epub 2006 May 11.
10
Salmonella typhi contains identical intervening sequences in all seven rrl genes.伤寒沙门氏菌在所有七个rrl基因中都含有相同的间隔序列。
J Bacteriol. 1996 Sep;178(17):5323-6. doi: 10.1128/jb.178.17.5323-5326.1996.
引用本文的文献
1
Comprehensive analysis of insertion sequences within rRNA genes of CPR bacteria and biochemical characterization of a homing endonuclease encoded by these sequences.CPR 细菌中 rRNA 基因内插入序列的综合分析及这些序列编码的归巢内切酶的生化特性。
J Bacteriol. 2024 Jul 25;206(7):e0007424. doi: 10.1128/jb.00074-24. Epub 2024 Jun 10.
2
Ancestry of RNA/RNA interaction regions within segmented ribosomes.分段核糖体中 RNA/RNA 相互作用区域的起源。
RNA. 2023 Sep;29(9):1388-1399. doi: 10.1261/rna.079654.123. Epub 2023 Jun 1.
3
mRNA extraction of Xanthomonas fragariae in strawberry and validation of reference genes for the RT-qPCR for study of bacterial gene expression.从草莓中提取黄单胞菌的 mRNA 并验证 RT-qPCR 研究细菌基因表达的内参基因。
Mol Biol Rep. 2019 Dec;46(6):5723-5733. doi: 10.1007/s11033-019-05006-z. Epub 2019 Jul 31.
4
Solithromycin inhibition of protein synthesis and ribosome biogenesis in Staphylococcus aureus, Streptococcus pneumoniae, and Haemophilus influenzae.索利霉素对金黄色葡萄球菌、肺炎链球菌和流感嗜血杆菌中蛋白质合成和核糖体生物发生的抑制作用。
Antimicrob Agents Chemother. 2013 Apr;57(4):1632-7. doi: 10.1128/AAC.02316-12. Epub 2013 Jan 14.
5
Structure of the essential diversity-generating retroelement protein bAvd and its functionally important interaction with reverse transcriptase.必需的多样性生成逆转录元件蛋白 bAvd 的结构及其与逆转录酶的功能重要相互作用。
Structure. 2013 Feb 5;21(2):266-76. doi: 10.1016/j.str.2012.11.016. Epub 2012 Dec 27.
6
Identification and characterization of intervening sequences within 23S rRNA genes from more than 200 Campylobacter isolates from seven species including atypical campylobacters.鉴定和分析来自 7 个种（包括非典型弯曲菌）的 230 多个弯曲杆菌分离株 23S rRNA 基因内的插入序列。
BMC Microbiol. 2009 Dec 11;9:256. doi: 10.1186/1471-2180-9-256.
7
Molecular characterization of intervening sequences in 23S rRNA genes and 23S rRNA fragmentation in Taylorella equigenitalis.马泰勒氏菌23S rRNA基因间隔序列的分子特征及23S rRNA片段化分析
Folia Microbiol (Praha). 2008;53(6):486-92. doi: 10.1007/s12223-008-0076-0. Epub 2009 Apr 18.
8
Intragenomic 16S rDNA divergence in Haloarcula marismortui is an adaptation to different temperatures.盐沼盐杆菌基因组内16S rDNA的差异是对不同温度的一种适应。
J Mol Evol. 2007 Dec;65(6):687-96. doi: 10.1007/s00239-007-9047-3. Epub 2007 Nov 17.
9
The absence of intervening sequences in 23S rRNA genes of Campylobacter coli isolates from Turkeys is a unique attribute of a cluster of related strains which also lack resistance to erythromycin.来自火鸡的空肠弯曲菌分离株的23S rRNA基因中不存在间隔序列，这是一组相关菌株的独特特征，这些菌株对红霉素也没有抗性。
Appl Environ Microbiol. 2007 Feb;73(4):1208-14. doi: 10.1128/AEM.01995-06. Epub 2006 Dec 22.
10
Limited role for the DsrA and RprA regulatory RNAs in rpoS regulation in Salmonella enterica.DsrA和RprA调控RNA在肠炎沙门氏菌rpoS调控中的作用有限。
J Bacteriol. 2006 Jul;188(14):5077-88. doi: 10.1128/JB.00206-06.
本文引用的文献
1
Sequence diversity of intervening sequences (IVSs) in the 23S ribosomal RNA in Salmonella spp.沙门氏菌属23S核糖体RNA中插入序列（IVSs）的序列多样性
Gene. 2000 Jul 25;253(1):55-66. doi: 10.1016/s0378-1119(00)00239-0.
2
Distribution of intervening sequences in the genes for 23S rRNA and rRNA fragmentation among strains of the Salmonella reference collection B (SARB) and SARC sets.沙门氏菌参考菌株集B（SARB）和SARC组中23S rRNA基因间序列的分布及rRNA片段化情况。
J Bacteriol. 2000 Apr;182(7):1923-9. doi: 10.1128/JB.182.7.1923-1929.2000.
3
Fragmentation of 23S rRNA in strains of Proteus and Providencia results from intervening sequences in the rrn (rRNA) genes.变形杆菌属和普罗威登斯菌属菌株中23S rRNA的片段化是由rrn（rRNA）基因中的间隔序列导致的。
J Bacteriol. 2000 Feb;182(4):1109-17. doi: 10.1128/JB.182.4.1109-1117.2000.
4
The phylogenetic position of Serratia, Buttiauxella and some other genera of the family Enterobacteriaceae.沙雷氏菌属、布蒂亚氏菌属及肠杆菌科其他一些属的系统发育位置。
Int J Syst Bacteriol. 1999 Oct;49 Pt 4:1433-8. doi: 10.1099/00207713-49-4-1433.
5
Fragmentation heterogeneity of 23S ribosomal RNA in Haemophilus species.嗜血杆菌属中23S核糖体RNA的片段化异质性
Gene. 1999 Apr 16;230(2):287-93. doi: 10.1016/s0378-1119(99)00063-3.
6
Sequences around the fragmentation sites of the large subunit ribosomal RNA in the family Rhizobiaceae. 23S-like rRNAs in Rhizobiaceae.根瘤菌科中大亚基核糖体RNA断裂位点周围的序列。根瘤菌科中的23S样rRNA。
Antonie Van Leeuwenhoek. 1998 Jan;73(1):55-67. doi: 10.1023/a:1000540023194.
7
RNase III deficient Salmonella typhimurium LT2 contains intervening sequences (IVSs) in its 23S rRNA.核糖核酸酶III缺陷型鼠伤寒沙门氏菌LT2在其23S核糖体RNA中含有间隔序列（IVSs）。
FEMS Microbiol Lett. 1998 Feb 15;159(2):179-85. doi: 10.1111/j.1574-6968.1998.tb12858.x.
8
Sequence similarities between large subunit ribosomal RNA gene intervening sequences from different Helicobacter species.不同幽门螺杆菌物种的大亚基核糖体RNA基因间隔序列之间的序列相似性。
Gene. 1997 Jul 18;194(1):69-75. doi: 10.1016/s0378-1119(97)00158-3.
9
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.空位BLAST和位置特异性迭代BLAST：新一代蛋白质数据库搜索程序。
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402. doi: 10.1093/nar/25.17.3389.
10
Functional Escherichia coli 23S rRNAs containing processed and unprocessed intervening sequences from Salmonella typhimurium.含有来自鼠伤寒沙门氏菌已加工和未加工间隔序列的功能性大肠杆菌23S rRNA。
Nucleic Acids Res. 1996 Dec 15;24(24):4918-23. doi: 10.1093/nar/24.24.4918.
Zotero 插件