Pronk L M, Sanderson K E
Salmonella Genetic Stock Centre, Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada T2N 1N4.
J Bacteriol. 2001 Oct;183(19):5782-7. doi: 10.1128/JB.183.19.5782-5787.2001.
Intervening sequences (IVSs) in the rrl genes for 23S rRNA are transcribed but later removed by RNase III without religation during RNA processing, leading to fragmented rRNA. We examined about 240 strains of the family Enterobacteriaceae for presence of IVSs using PCR. No IVSs were detected in strains belonging to Escherichia, Shigella, Enterobacter, Erwinia, Ewingella, Hafnia, Kluyvera, Morganella, Pantoea, or Serratia. Previously unreported IVSs were detected in Klebsiella oxytoca, Citrobacter amalonaticus, and Providencia stuartii; previously reported IVSs are in species of Salmonella, Proteus, Providencia, and Yersinia. The sporadic distribution of IVSs indicates lateral genetic transfer of IVSs.
23S rRNA的rrl基因中的间隔序列（IVSs）会被转录，但在RNA加工过程中随后会被RNase III去除且不会重新连接，从而导致rRNA片段化。我们使用PCR检测了约240株肠杆菌科细菌中IVSs的存在情况。在属于大肠杆菌、志贺氏菌、肠杆菌、欧文氏菌、尤因氏菌、哈夫尼亚菌、克吕韦氏菌、摩根氏菌、泛菌或沙雷氏菌的菌株中未检测到IVSs。在产酸克雷伯菌、无丙二酸柠檬酸杆菌和斯氏普罗威登斯菌中检测到了先前未报道的IVSs；先前报道的IVSs存在于沙门氏菌、变形杆菌、普罗威登斯菌和耶尔森氏菌的物种中。IVSs的零星分布表明IVSs存在横向基因转移。
