[肽免疫调节剂甘利欣对人白细胞氧化和抗氧化系统中假结核耶尔森菌耐热毒素活性的影响]

[Effect of peptide immunomodulator gangleen on the activity of Yersinia pseudotuberculosis thermostable toxin in oxidant and antioxidant systems of human leukocytes].

作者信息

Logvinenko A A, Dolmatova L S, Timchenko N F, Epshteĭn L M

机构信息

Research Institute of Epidemiology and Microbiology, Siberian Branch of the Russian Academy of Medical Sciences, Pacific Ocean Institute, Pacific Ocean Research Institute of Fish Industry and Oceanography, Vladivostok.

出版信息

Antibiot Khimioter. 2001;46(2):17-21.

PMID:11544746

Abstract

The ability to correct activity of Y. pseudotuberculosis thermoresistant toxin on antioxidative enzymes and on active oxygen forms in neurophiles and mononuclears was investigated. Toxin at concentration 0.5 and 2.5 mcg/ml did not change O2- production and activity of superoxidedismutase (SOD) and glutathione peroxidase (GP), but significantly enhanced catalase and glutathiont reductase (GR) activity. Gangleen at concentration 0.0002-0.2 mcg/ml, when added to incubation medium with toxin, stimulated production of active oxygen forms and activity of SOD, catalase, GP in both types of leucocytes, but decreased activity of GR in mononuclears. The results of investigation proves the ability to use gangleen in correction of immune system disorders caused by Y. pseudotuberculosis thermoresistant toxin.

摘要

研究了校正假结核耶尔森氏菌耐热毒素对嗜神经细胞和单核细胞中抗氧化酶及活性氧形式活性的能力。浓度为0.5和2.5微克/毫升的毒素未改变超氧阴离子生成以及超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GP）的活性，但显著增强了过氧化氢酶和谷胱甘肽还原酶（GR）的活性。浓度为0.0002 - 0.2微克/毫升的钩吻素子添加到含毒素的孵育培养基中时，刺激了两种类型白细胞中活性氧形式的产生以及SOD、过氧化氢酶、GP的活性，但降低了单核细胞中GR的活性。研究结果证明了使用钩吻素子校正由假结核耶尔森氏菌耐热毒素引起的免疫系统紊乱的能力。