Caraux J, Dimitriu A, Dy M, Mellor J, Hamburger J
Transplantation. 1975 May;19(5):369-72. doi: 10.1097/00007890-197505000-00002.
Spleen cells from a C57BL/6 mouse allografted with DBA/2 skin may release a macrophage arming factor when stimulated with phytohemagglutinin. This in vitro nonspecific release is observed only when the recipient cells are collected during a limited period preceding or coinciding with graft rejection. The phenomenon disappears if the skin allograft has been removed before cell collection. It appears if an i.v. injection of donor cells is given to the recipient after graft removal, on the day preceding cell collection. These data suggest that this in vitro apparently nonspecific macrophage arming factor release by phytohemagglutinin-stimulated recipient cells may in fact disclose a previous specific in vivo immune cell triggering by graft antigens.
用DBA/2皮肤同种异体移植的C57BL/6小鼠的脾细胞在用植物血凝素刺激时可能释放巨噬细胞武装因子。仅当在移植排斥之前或与之同时的有限时间段内收集受体细胞时，才会观察到这种体外非特异性释放。如果在细胞收集之前已切除皮肤同种异体移植物，该现象就会消失。如果在移植物切除后、细胞收集前一天给受体静脉注射供体细胞，该现象就会出现。这些数据表明，植物血凝素刺激的受体细胞在体外明显非特异性释放巨噬细胞武装因子，实际上可能揭示了先前移植物抗原在体内对免疫细胞的特异性触发。
