Jungnickel M K, Hinds L A
Cooperative Research Centre for Conservation and Management of Marsupials, Department of Biological Sciences, University of Newcastle, New South Wales, Australia.
Reprod Fertil Dev. 2000;12(7-8):457-64. doi: 10.1071/rd99037.
This study investigated the effect of superovulation with exogenous porcine FSH/LH on the normal hormonal milieu of the tammar wallaby (Macropus eugenii). During seasonal and lactational quiescence, groups of 6 females were treated with either multiple doses of porcine follicle-stimulating hormone (FSH) (8 x 6 mg i.m., 12 h apart) followed by a single subcutaneous injection of 4 mg porcine luteinizing hormone (LH) on Day 5 or saline. Blood samples were collected throughout each 10-day experimental period and each female was examined twice daily for signs of a recent copulation. On Day 9, females were killed and their reproductive tracts removed for examination and flushed for eggs. During both seasonal and lactational quiescence, treatment with porcine FSH/LH induced circulating concentrations of progesterone, porcine FSH and porcine LH that were within the normal range of the natural tammar oestrous cycle. However, higher plasma oestradiol concentrations (30-50 microg mL(-1)) than would be expected in a natural tammar preovulatory rise and the presence of 'highly stimulated' ovaries in several of the treated animals suggests that some degree of over-stimulation was occurring. During both seasonal sampling periods, behavioural oestrus was detected in treated tammars in the absence of a withdrawal of progesterone. This data suggests that plasma progesterone is not the critical factor inducing behavioural oestrus.
本研究调查了外源性猪促卵泡素/促黄体素超数排卵对帚尾袋貂（Macropus eugenii）正常激素环境的影响。在季节性静止期和哺乳期静止期，将6只雌性帚尾袋貂分为两组，一组多次注射猪促卵泡素（FSH）（8×6mg，肌肉注射，间隔12小时），并在第5天皮下注射一次4mg猪促黄体素（LH），另一组注射生理盐水。在整个10天的实验期内采集血样，每天对每只雌性帚尾袋貂检查两次，观察近期交配迹象。在第9天，处死雌性帚尾袋貂，取出其生殖道进行检查并冲洗以获取卵子。在季节性静止期和哺乳期静止期，用猪促卵泡素/促黄体素处理均诱导了孕酮、猪促卵泡素和猪促黄体素的循环浓度，这些浓度处于帚尾袋貂自然发情周期的正常范围内。然而，处理后的几只动物血浆雌二醇浓度（30 - 50μg mL⁻¹）高于帚尾袋貂自然排卵前预期的升高水平，并且出现了“过度刺激”的卵巢，这表明存在一定程度的过度刺激。在两个季节性采样期内，在处理后的帚尾袋貂中均检测到行为性发情，且孕酮未出现下降。该数据表明血浆孕酮不是诱导行为性发情的关键因素。
