Muhvić D, El-Samalouti V, Flad H D, Radosević-Stasić B, Rukavina D
Department of Physiology and Immunology, Medical Faculty, University of Rijeka, Croatia.
Mediators Inflamm. 2001 Jun;10(3):155-62. doi: 10.1080/09629350123956.
Cell-wall components of Gram-positive and Gram-negative bacteria induce the production of cytokines in human peripheral blood mononuclear cells. These cytokines are the main mediators of local or systemic inflammatory reaction that can contribute to the development of innate immunity.
This study was performed to analyze the involvement of CD14 molecule in the activation of human monocytes by peptidoglycan monomer (PGM) obtained by biosynthesis from culture fluid of penicillin-treated Brevibacterium divaricatum NRLL-2311.
Cytokine release of interleukin (IL)-1, IL-6 and tumor necrosis factor-alpha from human monocytes via soluble CD14 (sCD14) or membrane-associated (mCD14) receptor using anti-CD14 monoclonal antibody (MEM-18) or lipid A structure (compound 406) was measured in bioassays.
The results demonstrated that PGM in the presence of human serum might induce the monokine release in a dose-dependent manner. The addition of sCD14 at physiologic concentrations enhanced the PGM-induced monokine release, while the monokine inducing capacity of PGM in the presence of sCD14 was inhibited by MEM-18. Effects of PGM were also blocked by glycolipid, compound 406, suggesting the involvement of binding structures similar to those for lipopolysaccharide.
Activation of human monocytes by PGM involves both forms of CD14 molecule, sCD14 and mCD14.
革兰氏阳性菌和革兰氏阴性菌的细胞壁成分可诱导人外周血单核细胞产生细胞因子。这些细胞因子是局部或全身炎症反应的主要介质，可促进固有免疫的发展。
本研究旨在分析CD14分子在由青霉素处理的分叉短杆菌NRLL-2311培养液经生物合成获得的肽聚糖单体（PGM）激活人单核细胞过程中的作用。
在生物测定中，使用抗CD14单克隆抗体（MEM-18）或脂多糖A结构（化合物406），通过可溶性CD14（sCD14）或膜相关（mCD14）受体测量人单核细胞释放白细胞介素（IL）-1、IL-6和肿瘤坏死因子-α的细胞因子水平。
结果表明，在人血清存在的情况下，PGM可能以剂量依赖的方式诱导单核因子释放。生理浓度的sCD14的添加增强了PGM诱导的单核因子释放，而在sCD14存在的情况下，PGM的单核因子诱导能力被MEM-18抑制。PGM的作用也被糖脂化合物406阻断，表明存在与脂多糖类似的结合结构。
PGM对人单核细胞的激活涉及sCD14和mCD14这两种形式的CD14分子。
