Wu S M, Ho Y H, Wu H L, Chen S H, Ko H S
School of pharmacy, College of Pharmacy, Kaohsiung Medical University, ROC, Taiwan.
Electrophoresis. 2001 Aug;22(13):2758-62. doi: 10.1002/1522-2683(200108)22:13<2758::AID-ELPS2758>3.0.CO;2-P.
A simple capillary zone electrophoresis (CZE) method is described for the simultaneous determination of cimetidine (CIM), famotidine (FAM), nizatidine (NIZ), and ranitidine (RAN). The analysis of these drugs was performed in a 100 mM phosphate buffer, pH 3.5. Several parameters were studied, including wavelength for detection, concentration and pH of phosphate buffer, and separation voltage. The quantitative ranges were 100-1,000 microM for each analyte. The intra- and interday relative standard deviations (n = 5) were all less than 4%. The detection limits were found to be about 10 microM for CIM, 20 microM for RAN, 20 microM for NIZ, and 10 microM for FAM (S/N = 3, injection 1 s) at 214 nm. All recoveries were greater than 92%. Applications of the method to the assay of these drugs in tablets proved to be feasible.
描述了一种简单的毛细管区带电泳（CZE）方法，用于同时测定西咪替丁（CIM）、法莫替丁（FAM）、尼扎替丁（NIZ）和雷尼替丁（RAN）。这些药物的分析在pH 3.5的100 mM磷酸盐缓冲液中进行。研究了几个参数，包括检测波长、磷酸盐缓冲液的浓度和pH值以及分离电压。每种分析物的定量范围为100 - 1000 microM。日内和日间相对标准偏差（n = 5）均小于4%。在214 nm处，CIM的检测限约为10 microM，RAN为20 microM，NIZ为20 microM，FAM为10 microM（S/N = 3，进样1 s）。所有回收率均大于92%。该方法应用于片剂中这些药物的测定被证明是可行的。
