Błasiak J
Department of Molecular Genetics, University of Lódź, Poland.
Cytobios. 2001;106 Suppl 2:141-9.
Alcohol intake may be associated with various diseases including cancer. Defective DNA repair may underline neoplastic transformation, so it is reasonable to investigate the potential influence of ethanol on this process. In the present work the repair of DNA damaged by an antitumour drug bleomycin in human lymphocytes in the presence of ethanol or its main metabolite, acetaldehyde, was investiagted using single cell gel electrophoresis (comet assay). The cells were incubated with bleomycin at 1.5 or 5 microg/ml for 60 min at 37 degrees C, washed and suspended in a bleomycin-free medium containing ethanol at 10 or 30 mM or acetaldehyde at 1 or 3 mM. Ethanol at 30 mM and acetaldehyde inhibited the repair of DNA exposed to bleomycin at 5 microg/ml. These results suggest that ethanol and acetaldehyde may influence the repair of carcinogen-damaged DNA and thus contribute to cancer.
酒精摄入可能与包括癌症在内的多种疾病有关。DNA修复缺陷可能是肿瘤转化的基础，因此研究乙醇对这一过程的潜在影响是合理的。在本研究中，使用单细胞凝胶电泳（彗星试验）研究了在乙醇或其主要代谢产物乙醛存在的情况下，抗肿瘤药物博来霉素对人淋巴细胞中DNA损伤的修复情况。将细胞与1.5或5微克/毫升的博来霉素在37℃下孵育60分钟，洗涤后悬浮于不含博来霉素但含有10或30毫摩尔乙醇或1或3毫摩尔乙醛的培养基中。30毫摩尔的乙醇和乙醛抑制了暴露于5微克/毫升博来霉素的DNA的修复。这些结果表明，乙醇和乙醛可能会影响致癌物损伤的DNA的修复，从而导致癌症。
