Kolon T F, Miller O F
Department of Urology, Naval Medical Center, San Diego, California, USA.
J Urol. 2001 Oct;166(4):1451-4.
Human chorionic gonadotropin (HCG) has a stimulatory effect on testicular steroidogenesis and has been widely used for evaluating male Leydig cell function. However, considerable variability exists in the protocols for HCG stimulation tests. In the most commonly used protocols HCG is administered daily for several days. We examine the circulating androgen response after 1 and 3-dose HCG regimens.
We evaluated 77 prepubertal boys diagnosed with hypospadias, cryptorchidism or micropenis. In 60 boys who underwent 1 dose of 100 IU/kg. or 5,000 IU/1.7 m.(2) HCG serum testosterone and dihydrotestosterone were sampled at 72 (28) and 96 (32) hours after injection, while in 17 who underwent 3 daily age adjusted doses hormone levels were determined on day 4. All blood specimens were obtained and injections were performed at 8:00 to 9:00 a.m. and all specimens were evaluated at the same endocrine reference laboratory.
Nonstimulated testosterone levels were prepubertal in all groups. In the 1-dose groups post-stimulation testosterone was elevated 22 to 29-fold from baseline after a weight based and 34 to 35-fold after a body surface area based dose. Testosterone increased 20-fold baseline in the multi-dose group. No significant differences were observed in 72 versus 96-hour hormone levels.
Evaluating Leydig cell function by HCG stimulation is an important adjunct to the diagnosis of various urological conditions. A single weight or body surface area based HCG dose with androgen measurement after 3 or 4 days is a practical, reliable and cost saving change in testicular evaluation.
人绒毛膜促性腺激素（HCG）对睾丸类固醇生成具有刺激作用，已被广泛用于评估男性睾丸间质细胞功能。然而，HCG刺激试验的方案存在很大差异。在最常用的方案中，HCG连续数天每日给药。我们研究了单剂量和三剂量HCG方案后的循环雄激素反应。
我们评估了77名诊断为尿道下裂、隐睾或小阴茎的青春期前男孩。在60名接受100IU/kg单剂量或5000IU/1.7m² HCG的男孩中，注射后72（28）和96（32）小时采集血清睾酮和双氢睾酮样本，而在17名接受每日3次年龄调整剂量的男孩中，在第4天测定激素水平。所有血样均在上午8:00至9:00采集，注射也在此时间段进行，所有样本均在同一内分泌参考实验室进行评估。
所有组的非刺激睾酮水平均处于青春期前。在单剂量组中，刺激后睾酮水平从基线升高了22至29倍（基于体重给药）和34至35倍（基于体表面积给药）。多剂量组中睾酮水平升高了20倍基线。72小时和96小时的激素水平未观察到显著差异。
通过HCG刺激评估睾丸间质细胞功能是诊断各种泌尿系统疾病的重要辅助手段。基于体重或体表面积的单剂量HCG给药，并在3或4天后测量雄激素，是一种实用、可靠且节省成本的睾丸评估方法。
