Fukushi K, Kato S, Antsuki T, Omura T
Department of Civil Engineering, Graduate School of Engineering, Tohoku University, Japan.
Water Sci Technol. 2001;44(2-3):453-9.
Six copper-binding microbial proteins were isolated from activated sludge cultures grown on media containing copper at various concentrations. Molecular weights among isolated proteins were ranged from 1.3k to 1 74k dalton. Isolated proteins were compared for their copper binding capabilities. Proteins isolated from cultures grown in the presence of copper in the growth media exhibited higher copper binding capabilities than those isolated from the culture grown in the absence of copper. The highest metal uptake of 61.23 (mol copper/mol protein) was observed by a protein isolated from a culture grown with copper at a concentration of 0.25 mM. This isolated protein (CBP2) had a molecular weight of 24k dalton. Other protein exhibited copper binding capability of 4.8-32.5 (mol copper/mol protein).
从在含有不同浓度铜的培养基上生长的活性污泥培养物中分离出六种铜结合微生物蛋白。分离出的蛋白质分子量范围为1.3k至174k道尔顿。比较了分离出的蛋白质的铜结合能力。从生长培养基中存在铜的培养物中分离出的蛋白质比从无铜培养物中分离出的蛋白质表现出更高的铜结合能力。从铜浓度为0.25 mM的培养物中分离出的一种蛋白质观察到最高金属摄取量为61.23（摩尔铜/摩尔蛋白质）。这种分离出的蛋白质（CBP2）分子量为24k道尔顿。其他蛋白质表现出4.8 - 32.5（摩尔铜/摩尔蛋白质）的铜结合能力。
