Sucan M K, Russell G F
Applied Food Biotechnology, Inc., O'Fallon, MO 63366, USA.
Adv Exp Med Biol. 2001;488:165-73. doi: 10.1007/978-1-4615-1247-9_14.
The methods used to isolate volatile compounds for GC analyses can cause profound effects on the quantitative and qualitative composition of the injected sample, and exert a great influence in the resultant bioactivity of volatiles. Especially with plant tissues like tomatoes, the isolation of volatile constituents using classical methods may yield results which are not representative of the chemicals present in the natural material. Headspace sampling methods may be advantageous in capturing the same volatile compounds emitted from tomatoes that are detected by the human nose. This study utilized an extremely sensitive dynamic headspace sampling with thermal desorption method to determine volatile components of fresh tomato juices. The method proved very sensitive for the isolation of tomato volatiles and concentrations of flavor compounds were much greater than related literature studies.
用于气相色谱分析的挥发性化合物分离方法可能会对注入样品的定量和定性组成产生深远影响,并对挥发性物质的最终生物活性产生重大影响。特别是对于像番茄这样的植物组织,使用传统方法分离挥发性成分可能会得到不能代表天然材料中存在的化学物质的结果。顶空采样方法在捕获人鼻检测到的番茄释放的相同挥发性化合物方面可能具有优势。本研究采用极其灵敏的热脱附动态顶空采样法来测定新鲜番茄汁的挥发性成分。该方法被证明对番茄挥发性物质的分离非常灵敏,风味化合物的浓度远高于相关文献研究。