Vosloo W, Bastos A D, Michel A, Thomson G R
Onderstepoort Veterinary Institute, Private Bag X05, Onderstepoort 0110, South Africa.
Rev Sci Tech. 2001 Aug;20(2):630-9. doi: 10.20506/rst.20.2.1295.
Genetic characterisation of two pathogens, namely foot and mouth disease (FMD) virus and Mycobacterium bovis, isolated from African buffalo (Syncerus caffer) in southern Africa was used to determine the origin of buffalo in situations where the source of infection was obscure. By determining the phylogenetic relatedness of various FMD virus isolates using partial sequencing of the main antigenic determinant, VP1, the origin of buffalo moved illegally to the non-endemic region of South Africa was traced to the Kruger National Park (KNP) where FMD is endemic in the buffalo population. Comparative analysis of the 'genetic fingerprints' of bovine tuberculosis isolates from buffalo and cattle has aided in tracing the original source of infection of buffalo populations in the KNP. Furthermore, these analyses have assisted in tracing the origin of infected animals that have been moved to other parts of South Africa.
对从非洲南部非洲水牛（非洲野水牛）中分离出的两种病原体，即口蹄疫（FMD）病毒和牛分枝杆菌进行基因特征分析，以确定在感染源不明的情况下水牛的来源。通过使用主要抗原决定簇VP1的部分测序来确定各种口蹄疫病毒分离株的系统发育相关性，追踪非法转移到南非非流行区的水牛的来源，发现其来自克鲁格国家公园（KNP），该地水牛群体中口蹄疫呈地方流行。对水牛和牛的牛结核分枝杆菌分离株的“基因指纹”进行比较分析，有助于追踪克鲁格国家公园水牛群体的原始感染源。此外，这些分析有助于追踪已转移到南非其他地区的感染动物的来源。
