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真空干燥细菌中功能的最佳保存(作者译)

[Optimal preservation of function in vacuum-dried bacteria (author's transl)].

作者信息

Eyer H, Schmidt M, Deffner G

出版信息

Zentralbl Bakteriol Orig A. 1975;231(1-3):243-9.

PMID:1154908
Abstract

Experience has shown that there are natural processes assuring a good preservation of virulence of delicate bacteria over intervals between epidemic outbreaks. Similarly, fetal bovine serum enriched with 10% saccharose was found to be an ideal medium for rendering bacteria well preservable without losing essential functional characteristics by means of vacuum-drying. Cotton wool DAB 7 has proved to be a good support. The available findings would let also delicate aerobic and anaerobic bacteria appear to be suitable for this kind of preservation, retaining their full capacity to multiply for at least one year inclusive of sensitive functional parameters. Results have been grouped in a number of synoptic tables, Bacteria that would not admit of the method of preservation described, have not been found thus far.

摘要

经验表明,在疫情爆发的间隔期,存在自然过程确保脆弱细菌的毒力得到良好保存。同样,发现富含10%蔗糖的胎牛血清是一种理想的培养基,通过真空干燥可使细菌易于保存且不丧失基本功能特性。脱脂棉DAB 7已被证明是一种良好的载体。现有研究结果表明,脆弱的需氧菌和厌氧菌似乎也适合这种保存方式,至少一年内它们能保持充分的繁殖能力,包括敏感的功能参数。结果已汇总在一些一览表中。迄今为止,尚未发现不适用所述保存方法的细菌。

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