Kabat I A, Sysa J, Leyko W, Kwiatkowski B, Sysa A, Zakrezewska I, Hłyńczak A
Zentralbl Bakteriol Orig B. 1975 May;160(3):225-36.
Effect of UV-irradiation in vitro on metabolism of adenine nucleotides: ADP, ATP and AXP, osmotic properties and submicroscopic structure of nucleated pigeon red blood cell was investigated. Irradiation carried out for 60, 120, 180, 240 and 300 minutes. Hanau S-500 lamp with efficiency 4.34-10(8) erh/sec was used. A decrease of ATP content with a stimultaneous increase of ADP and AXP contents and a rather constant level of the sum of adenine compounds was observed. UV-irradiation caused a decrease of reversal of hemolysis and osmotic resistance to hypotonic NaCl solutions. An equivalent exchange of Na+ and K+ ion and an increase of hematocrit value, following UV-irradiation was observed. Electron microscope studies demonstrated changes of ultrastructure concerning both cell nucleus and thickness and granulation of cell membrane.
体外紫外线照射对腺嘌呤核苷酸(ADP、ATP和AXP)代谢、有核鸽红细胞渗透特性及亚显微结构的影响进行了研究。照射时间分别为60、120、180、240和300分钟。使用效率为4.34×10⁸尔格/秒的哈瑙S - 500灯。观察到ATP含量降低,同时ADP和AXP含量增加,且腺嘌呤化合物总和水平相当恒定。紫外线照射导致溶血逆转及对低渗NaCl溶液的渗透压抗性降低。观察到紫外线照射后Na⁺和K⁺离子的等量交换以及血细胞比容值增加。电子显微镜研究表明,细胞核以及细胞膜厚度和颗粒度的超微结构发生了变化。