长期评估隔离饲养诱导的大鼠前脉冲抑制缺陷
Long-term evaluation of isolation-rearing induced prepulse inhibition deficits in rats.
作者信息
Cilia J, Reavill C, Hagan J J, Jones D N
机构信息
Neuroscience Research, GlaxoSmithKline, Harlow, Essex, UK.
出版信息
Psychopharmacology (Berl). 2001 Jul;156(2-3):327-37. doi: 10.1007/s002130100786.
RATIONALE
Rats reared in social isolation from weaning show prepulse inhibition (PPI) deficits which are thought to model the sensorimotor gating deficits seen in schizophrenia and other psychiatric disorders. However, recent studies have questioned the robustness of this paradigm.
OBJECTIVE
The existence of a substantial dataset generated over 4 years in our laboratory has allowed the investigation of the robustness and reliability of the procedure under a variety of environmental conditions. The effects of atypical antipsychotics (clozapine, olanzapine and risperidone) under different experimental conditions are also reported.
METHOD
At weaning, Hooded Lister pups were singly (isolates) or group (n=5) housed (grouped). Eight weeks later, the startle and PPI response of isolates and grouped rats was investigated using conditions of fixed inter-stimulus interval (ISI) (pulse=110 dB/50 ms; prepulse=80 dB/30 ms; ISI=100 ms) or variable
理论依据
从断奶起就饲养在社会隔离环境中的大鼠表现出前脉冲抑制（PPI）缺陷，这种缺陷被认为可模拟精神分裂症和其他精神疾病中出现的感觉运动门控缺陷。然而，最近的研究对该范式的稳健性提出了质疑。
目的
我们实验室在4年多时间里生成了大量数据集，这使得我们能够在各种环境条件下研究该程序的稳健性和可靠性。本文还报告了非典型抗精神病药物（氯氮平、奥氮平和利培酮）在不同实验条件下的作用。
方法
断奶时，将带帽利斯特幼崽单独（隔离饲养）或成组（每组5只）饲养（分组饲养）。八周后，使用固定刺激间隔（ISI）条件（脉冲=110分贝/50毫秒；前脉冲=80分贝/30毫秒；ISI=100毫秒）或可变刺激间隔条件，研究隔离饲养和分组饲养大鼠的惊吓反应和前脉冲抑制反应。
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