Kerwin S M, Sun D, Kern J T, Rangan A, Thomas P W
Division of Medicinal Chemistry, College of Pharmacy, University of Texas at Austin, 78712, USA.
Bioorg Med Chem Lett. 2001 Sep 17;11(18):2411-4. doi: 10.1016/s0960-894x(01)00490-5.
We have examined a number of carbocyanine dyes for their ability to bind intramolecular G-quadruplex DNA structures (G4'-DNA) using a Taq polymerase stop assay. Of the five dyes examined, only one, N,N'-diethylthiacarbocyanine iodide (DTC), was found to bind to G4'-DNA. DTC was also the only dye found to inhibit human telomerase at 50 microM concentration.
我们使用Taq聚合酶终止试验，检测了多种碳菁染料结合分子内G-四链体DNA结构（G4'-DNA）的能力。在所检测的五种染料中，仅发现一种染料，即碘化N，N'-二乙基硫代碳菁（DTC），能够结合G4'-DNA。DTC也是唯一一种在50微摩尔浓度下能抑制人端粒酶的染料。
