Schubert K, Kaufmann G, Knöll R
Acta Biol Med Ger. 1975;34(2):167-72.
Estradiol-3-methylether, estrone-3-methylether, 17alpha-ethinyl-estradiol-3-methylether and other 17alpha-substituted estratrienes were 3-O-demethylated to free 3-hydroxy compounds by fermentation with Corynebacterium sp. A hydroxy groupp in position 6alpha or 6beta prevented the reaction. The opposite reaction, methylation of the 3-hydroxy group of estratrienes, was performed using Mycobacterium smegmatis. The substrate specifity of this methylation was low. Analogies of these microbial reactions to steroid metabolism in mammalian organism are discussed.
雌二醇 - 3 - 甲醚、雌酮 - 3 - 甲醚、17α - 乙炔基雌二醇 - 3 - 甲醚以及其他17α - 取代的雌三烯通过与棒状杆菌属进行发酵反应被3 - O - 去甲基化为游离的3 - 羟基化合物。6α或6β位的羟基会抑制该反应。相反的反应，即雌三烯3 - 羟基的甲基化反应，则使用耻垢分枝杆菌来进行。这种甲基化反应的底物特异性较低。文中讨论了这些微生物反应与哺乳动物体内类固醇代谢之间的相似性。
