Jung I Y, Choi B, Kum K Y, Yoo Y J, Yoon T C, Lee S J, Lee C Y
Department of Conservative Dentistry and Oral Science Research Center, Yonsei University, Seoul, Korea.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001 Sep;92(3):329-34. doi: 10.1067/moe.2001.117263.
Recent molecular approaches have revealed that fastidious organisms such as Bacteroides forsythus and oral treponemes were frequently found in root canals with apical periodontitis. The purpose of this study was to identify the isolates of oral spirochetes at the species level in endodontic infections and to determine their association with B forsythus and Porphyromonas gingivalis.
Seventy-nine teeth with apical periodontitis were selected for this study. After sampling from the root canals aseptically, polymerase chain reaction amplification for the 16S rRNA gene was performed with eubacterial universal primers. Subsequently, dot-blot hybridization was performed with 8 species-specific oligonucleotide probes. The microbial associations were analyzed by using the odds ratio.
The most frequently found species was P gingivalis (27.4%), followed by Treponema maltophilum (26%), B forsythus (16.4%), and Treponema socranskii (2.7%). Other treponemes, including Treponema denticola, were not detected in our samples. Significant microbial associations were identified between T maltophilum, B forsythus, and P gingivalis by performing analysis with the odds ratio.
Our results indicate that T maltophilum should be included in etiologic studies of endodontic diseases.
最近的分子生物学方法显示，在患有根尖周炎的根管中经常发现诸如福赛斯坦纳菌和口腔密螺旋体等苛求菌。本研究的目的是在牙髓感染中鉴定口腔螺旋体在种水平上的分离株，并确定它们与福赛斯坦纳菌和牙龈卟啉单胞菌的关系。
本研究选取了79颗患有根尖周炎的牙齿。在无菌条件下从根管取样后，使用真细菌通用引物对16S rRNA基因进行聚合酶链反应扩增。随后，用8种特异性寡核苷酸探针进行斑点杂交。通过比值比分析微生物之间的关联。
最常发现的菌种是牙龈卟啉单胞菌（27.4%），其次是嗜麦芽寡养单胞菌（26%）、福赛斯坦纳菌（16.4%）和索氏密螺旋体（2.7%）。在我们的样本中未检测到包括具核梭杆菌在内的其他密螺旋体。通过比值比分析，在嗜麦芽寡养单胞菌、福赛斯坦纳菌和牙龈卟啉单胞菌之间发现了显著的微生物关联。
我们的结果表明，嗜麦芽寡养单胞菌应纳入牙髓病病因学研究。
