Jung I Y, Choi B K, Kum K Y, Roh B D, Lee S J, Lee C Y, Park D S
Department of Conservative Dentistry, Yonsei University, College of Dentistry, 134 Shinchon-Dong Seodaemun-Ku, Seoul, Korea.
J Endod. 2000 Oct;26(10):599-604. doi: 10.1097/00004770-200010000-00006.
The purpose of this study was to investigate the epidemiology of putative pathogens in root canals with apical periodontitis and to determine the associations among the putative pathogens. Eighteen symptomatic and 20 asymptomatic teeth from 36 subjects were studied. This research was performed with polymerase chain reaction and hybridization using rRNA-based oligonucleotide probes. The most frequently found species was Fusobacterium sp. (68.4%), followed by Peptostreptococcus micros (44.7%) and Porphyromonas gingivalis (26.3%). Sixteen teeth (42.1%) contained one or more species of the selected black-pigmented bacteria. Bacteroides forsythus and Treponema sp. were detected in 8 teeth and 6 teeth, respectively. Among the analyzed bacteria, significant relationships were shown in the combination of B. forsythus/P. gingivalis and Treponema sp./P. gingivalis. There was no significant association between any bacteria and any symptoms.
本研究的目的是调查根尖周炎根管中假定病原体的流行病学情况，并确定这些假定病原体之间的关联。对来自36名受试者的18颗有症状牙齿和20颗无症状牙齿进行了研究。本研究采用基于rRNA的寡核苷酸探针进行聚合酶链反应和杂交。最常发现的菌种是具核梭杆菌（68.4%），其次是微小消化链球菌（44.7%）和牙龈卟啉单胞菌（26.3%）。16颗牙齿（42.1%）含有一种或多种所选的产黑色素细菌。福赛坦氏菌和密螺旋体分别在8颗牙齿和6颗牙齿中检测到。在分析的细菌中，福赛坦氏菌/牙龈卟啉单胞菌和密螺旋体/牙龈卟啉单胞菌的组合显示出显著关系。任何细菌与任何症状之间均无显著关联。
