恶臭假单胞菌细胞色素P-450cam与苯巴比妥诱导的兔肝微粒体细胞色素P-450lm的免疫化学及成分比较。
Immunochemical and compositional comparison of cytochrome P-450 cam of Pseudomonas putida and P-450 lm of phenobarbital-induced rabbit liver microsomes.
作者信息
Dus K, Litchfield W J, Miguel A G
出版信息
Adv Exp Med Biol. 1975;58(00):47-53. doi: 10.1007/978-1-4615-9026-2_3.
PMID:1155253
Abstract
Although highly purified cytochrome P-450 of Pseudomonas putida (P-450 cam), and that from phenobarbital-induced rabbit liver microsomes (P-450 LM), differ markedly in their catalytic and physical properties, they show immunological cross reaction by competitive binding and inhibition of catalytic activity, and are of similar amino acid composition. Upon treatment with cyanogen bromide they yield small heme-containing peptides of highly similar amino acid composition.
摘要
尽管恶臭假单胞菌的高度纯化细胞色素P-450（P-450cam）与苯巴比妥诱导的兔肝微粒体中的细胞色素P-450（P-450LM）在催化和物理性质上有显著差异，但它们通过竞争性结合和催化活性抑制表现出免疫交叉反应，并且氨基酸组成相似。用溴化氰处理后，它们会产生氨基酸组成高度相似的含血红素小肽。
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