肠致病性大肠杆菌束状菌毛在宿主细胞黏附和微菌落形成中的作用。

Role of bundle-forming pilus of enteropathogenic Escherichia coli in host cell adherence and in microcolony development.

作者信息

Tobe T, Sasakawa C

机构信息

Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan.

出版信息

Cell Microbiol. 2001 Sep;3(9):579-85. doi: 10.1046/j.1462-5822.2001.00136.x.

DOI:10.1046/j.1462-5822.2001.00136.x

PMID:11553010

Abstract

Enteropathogenic Escherichia coli (EPEC) adheres to epithelial cells and forms microcolonies in localized areas. Bundle-forming pili (BFP) are necessary for autoaggregation and the formation of microcolonies. In this study, we show that BFP, expressed by EPEC on epithelial cells, disappeared with the expansion of the microcolony. Bacterial dispersal and the release of BFP from the EPEC aggregates were induced by contact with host cellular membrane extract. In addition, BFP-expressing EPEC adhered directly to cell surfaces, in preference to attaching to pre-formed microcolonies on the cells. These results suggested that BFP mediate the initial attachment of EPEC through direct interaction with the host cell rather than through the recruitment of unattached bacteria to microcolonies on the cell.

摘要

肠致病性大肠杆菌（EPEC）粘附于上皮细胞并在局部区域形成微菌落。束状菌毛（BFP）对于自身聚集和微菌落的形成是必需的。在本研究中，我们发现EPEC在上皮细胞上表达的BFP随着微菌落的扩大而消失。与宿主细胞膜提取物接触可诱导细菌扩散以及BFP从EPEC聚集体中释放。此外，表达BFP的EPEC直接粘附于细胞表面，优先于附着在细胞上预先形成的微菌落。这些结果表明，BFP通过与宿主细胞的直接相互作用介导EPEC的初始粘附，而不是通过将未附着的细菌募集到细胞上的微菌落。