运用原子力显微镜（AFM）研究精索静脉曲张精子的超微结构病理学。

Ultrastructural pathology of varicocele spermatozoa by using atomic force microscopy (AFM).

作者信息

Joshi N V, Medina H, Osuna J A

机构信息

Laboratorio de Fisiologia de la Conducta, Facultad de Medicina, Universidad de Los Andes, Merida, Venezuela.

出版信息

Arch Androl. 2001 Apr-Jun;47(2):143-52. doi: 10.1080/014850101316901352.

DOI:10.1080/014850101316901352

PMID:11554686

Abstract

Ultrastructural investigation of pathological spermatozoa of patients having varicocele (grades I and III) in its natural environment (without fixation, dehydration, embedding, sectioning, etc.) was carried out by using Atomic Force Microscope (AFM) in tapping mode. This is a unique technique to examine the topographical structure with precision and to obtain 3-D images. It revealed structural alterations caused by the presence of varicocele. Not only neckpieces and flagella were defective, but also the head; particularly, dimensions and heights are altered.

摘要

在自然环境下（不经过固定、脱水、包埋、切片等处理），利用原子力显微镜（AFM）的轻敲模式对患有精索静脉曲张（I级和III级）患者的病理精子进行了超微结构研究。这是一种能够精确检查地形结构并获取三维图像的独特技术。研究揭示了精索静脉曲张导致的结构改变。不仅颈部和鞭毛存在缺陷，头部也有问题；特别是尺寸和高度发生了变化。