含6-三氟甲基嘌呤核糖核苷的RNA的合成与分析

Synthesis and analysis of RNA containing 6-trifluoromethylpurine ribonucleoside.

作者信息

Véliz E A, Stephens O M, Beal P A

机构信息

Department of Chemistry, University of Utah, Salt Lake City, UT 84112, USA.

出版信息

Org Lett. 2001 Sep 20;3(19):2969-72. doi: 10.1021/ol016295i.

DOI:10.1021/ol016295i

PMID:11554820

Abstract

We report the synthesis of a 5'-DMT-2'-TBDMS-protected phosphoramidite of 6-trifluoromethylpurine ribonucleoside ((TFM)P) and its use in the site-specific incorporation of 6-trifluoromethylpurine into RNA. Properties of (TFM)P-substituted RNA suggest it will be valuable in the study of RNA structure and the binding of RNA-modifying enzymes, particularly the RNA-editing adenosine deaminases. Reaction: see text.

摘要

我们报道了6-三氟甲基嘌呤核糖核苷（(TFM)P）的5'-二甲氧基三苯甲基-2'-叔丁基二甲基硅烷基保护的亚磷酰胺的合成及其在将6-三氟甲基嘌呤位点特异性掺入RNA中的应用。(TFM)P取代的RNA的性质表明，它在RNA结构研究和RNA修饰酶（特别是RNA编辑腺苷脱氨酶）的结合研究中将具有重要价值。反应：见正文。