在紫外线B诱导的人黑色素瘤细胞系培养物凋亡中，Bcl-2的下调和半胱天冬酶-8的激活。
Downregulation of Bcl-2 and activation of caspase-8 in the UVB-induced apoptosis of a cultured human melanoma cell line.
作者信息
Park K H, Choi H O, Jang D D, Park Y I, Park K C
机构信息
Graduate School of Biotechnology, Korea University, Seoul, Korea.
出版信息
Photodermatol Photoimmunol Photomed. 2001 Oct;17(5):218-22. doi: 10.1034/j.1600-0781.2001.170503.x.
PURPOSE
This study was performed to determine the effect of UV radiation on the activation of apoptosis regulatory proteins using cultured human melanoma cells.
METHODS
G361 lightly pigmented melanoma cells were irradiated with increasing doses of UVB and analyzed for an apoptotic mechanism using a cell viability test, TEM, FACS, and western blotting analysis.
RESULTS
TEM and FACS showed apoptotic features of cell death after UVB irradiation. Western blotting disclosed downregulation of Bcl-2 and the activation of caspase-9. Caspase-8, a downstream molecule of Fas/FasL interaction, was also activated. The activation of downstream molecules of both caspase-8 and caspase-9 was also demonstrated.
CONCLUSION
Our data showed that the regulation of the Bcl-2 family and caspase-8 may work together to activate a caspase-3 mediated apoptotic pathway following UVB irradiation of cultured human melanoma cells.
目的
本研究旨在利用培养的人黑色素瘤细胞确定紫外线辐射对凋亡调节蛋白激活的影响。
方法
用递增剂量的中波紫外线（UVB）照射色素较浅的G361黑色素瘤细胞，并使用细胞活力测试、透射电子显微镜（TEM）、荧光激活细胞分选术（FACS）和蛋白质免疫印迹分析对凋亡机制进行分析。
结果
TEM和FACS显示UVB照射后细胞死亡的凋亡特征。蛋白质免疫印迹显示Bcl-2下调以及半胱天冬酶-9激活。Fas/FasL相互作用的下游分子半胱天冬酶-8也被激活。半胱天冬酶-8和半胱天冬酶-9的下游分子激活也得到证实。
结论
我们的数据表明，Bcl-2家族和半胱天冬酶-8的调节可能共同作用，在培养的人黑色素瘤细胞经UVB照射后激活半胱天冬酶-3介导的凋亡途径。
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