Moreno E C, Varughese K, Hay D I
Calcif Tissue Int. 1979 Aug 24;28(1):7-16. doi: 10.1007/BF02441212.
Inhibition of calcium phosphate precipitation in saliva, and prevention of the formation of mineral accretions on tooth surfaces, has been ascribed to the existence of inhibiting salivary macromolecules. Marked reductions in the crystal growth rate of hydroxyapatite (HA) seeds were measured in supersaturated solutions containing either of two proline-rich proteins, PRP1 or PRP3, or statherin; the three macromolecules were isolated from human parotid saliva. The reductions were also observed when the HA seeds were pretreated with solutions of the macromolecules before adding them to the supersaturated calcium phosphate solution. This effect was very similar in the case of the two PRPs and it was directly related to the extent of adsorption site coverage of these proteins on the HA seeds. The effect of statherin was larger than anticipated from its adsorption behavior. However, comparison on the basis of number of moles adsorbed per unit area of HA shows that the PRP are more effective inhibitors than statherin. The macromolecule concentrations used were considerably lower than those in the salivary secretions, therefore these macromolecules could readily prevent mineral accretion on tooth surfaces through their adsorption onto the enamel surface.
唾液中磷酸钙沉淀的抑制以及牙齿表面矿物质沉积物形成的预防，归因于抑制性唾液大分子的存在。在含有两种富含脯氨酸的蛋白质（PRP1或PRP3）或磷蛋白的过饱和溶液中，测量到羟基磷灰石（HA）晶种的晶体生长速率显著降低；这三种大分子是从人腮腺唾液中分离出来的。当将HA晶种用大分子溶液预处理后再加入过饱和磷酸钙溶液中时，也观察到了这种降低。两种PRP的情况非常相似，并且这与这些蛋白质在HA晶种上的吸附位点覆盖程度直接相关。磷蛋白的作用比根据其吸附行为预期的要大。然而，基于每单位面积HA吸附的摩尔数进行比较表明，PRP比磷蛋白是更有效的抑制剂。所使用的大分子浓度远低于唾液分泌物中的浓度，因此这些大分子可以通过吸附到牙釉质表面而很容易地防止牙齿表面的矿物质沉积。
