Isola M, Cabras T, Inzitari R, Lantini M S, Proto E, Cossu M, Riva A
Department of Cytomorphology, University of Cagliari, Italy.
J Anat. 2008 May;212(5):664-8. doi: 10.1111/j.1469-7580.2008.00888.x. Epub 2008 Apr 8.
In order to increase current knowledge regarding statherin secretion into the oral cavity, ultrastructural localization of this peptide was investigated in human salivary glands by using a post-embedding immunogold staining technique. Statherin reactivity was found inside the granules of serous cells of parotid and submandibular glands. In parotid granules immunostaining was preferentially present in the less electron-dense region, whereas in submandibular serous granules the reactivity was uniform and the dense core always stained. By contrast, none or weak reactivity was observed in serous cells of major sublingual glands. These findings reveal for the first time the subcellular localization of statherin by electron transmission microscopy and confirm that of the three major types of salivary glands, the parotid and submandibular glands are the greatest source of salivary statherin. Moreover, they suggest that more than one packaging mechanism may be involved in the storage of statherin within serous granules of salivary glands.
为了增加目前关于牙本质磷蛋白分泌至口腔的知识，利用包埋后免疫金染色技术对人唾液腺中该肽进行超微结构定位研究。在腮腺和下颌下腺的浆液性腺泡细胞的颗粒内发现了牙本质磷蛋白反应性。在腮腺颗粒中，免疫染色优先出现在电子密度较低的区域，而在下颌下腺浆液性腺泡颗粒中，反应性是均匀的，致密核心总是被染色。相比之下，在主要舌下腺的浆液性腺泡细胞中未观察到或仅观察到微弱的反应性。这些发现首次通过电子透射显微镜揭示了牙本质磷蛋白的亚细胞定位，并证实了在三种主要类型的唾液腺中，腮腺和下颌下腺是唾液牙本质磷蛋白的最大来源。此外，这些发现表明，唾液腺浆液性腺泡颗粒内牙本质磷蛋白的储存可能涉及不止一种包装机制。
