Muruvi W, Hamudikuwanda H, Chakoma C, Kusina N T
Henderson Research Station, Mazowe, Zimbabwe.
Trop Anim Health Prod. 2001 Oct;33(5):431-40. doi: 10.1023/a:1010599924569.
The objective of the experiment was to compare the reproductive post-partum performance of beef cows synchronized for oestrus using prostaglandin F2alpha (PGF2alpha) alone or with a gonadotrophin-releasing hormone (GnRH)-based drug. Fifty-five post-partum lactating Tuli cows were randomly allocated to three groups. Two groups were synchronized using either two injections of PGF2alpha (500 microg Prosolvin per injection) given 11 days apart (group 1), or GnRH (12.5 microg Receptal per injection) followed 6 days later by an injection of 500 microg PGF2alpha (group 2). The cows were bred by artificial insemination 12 h after they were observed in oestrus. Group 3 was synchronized as for group 2, but a second injection of GnRH was given 54 h after the PGF2alpha injection, at which time the cows were bred by artificial insemination (AI) without detection of oestrus. Blood samples were taken from the cows in group 3 and analysed for progesterone concentration to establish which cows were cycling and in oestrus before and at the time of breeding. Detection of oestrus and breeding by AI was done over 60 days. There were no significant differences (p>0.05) among the three groups in the first service and total conception rates. The percentage of cows in oestrus within 10 days of the synchronization treatment was not significantly different (p>0.05) between groups 1 and 2. The progesterone concentrations in the cows in group 3 showed that only those that were cycling at the start of the experiment responded to the synchronization treatment and conceived after fixed-time breeding. These results suggest that combinations of PGF2alpha and GnRH may be of value in synchronizing oestrus and controlling breeding in Tuli cows. However, the benefit might be greater if only cows that are known to be cycling are bred in this way.
该实验的目的是比较单独使用前列腺素F2α(PGF2α)或与基于促性腺激素释放激素(GnRH)的药物同步发情的肉牛产后繁殖性能。55头产后泌乳的图利奶牛被随机分为三组。两组分别采用以下方法进行同步发情处理:一组每隔11天注射两次PGF2α(每次注射500微克氯前列醇,第1组);另一组先注射GnRH(每次注射12.5微克瑞普太林),6天后再注射500微克PGF2α(第2组)。奶牛在发情后12小时进行人工授精。第3组的同步发情处理与第2组相同,但在注射PGF2α后54小时再注射一次GnRH,此时奶牛不进行发情检测直接进行人工授精。采集第3组奶牛的血样,分析孕酮浓度,以确定哪些奶牛在配种前和配种时处于发情周期。在60天内进行发情检测和人工授精。三组的首次输精受胎率和总受胎率没有显著差异(p>0.05)。第1组和第2组在同步发情处理后10天内发情奶牛的百分比没有显著差异(p>0.05)。第3组奶牛的孕酮浓度表明,只有那些在实验开始时处于发情周期的奶牛对同步发情处理有反应,并在定时配种后受孕。这些结果表明,PGF2α和GnRH的组合在图利奶牛发情同步化和繁殖控制方面可能具有价值。然而,如果仅对已知处于发情周期的奶牛采用这种方法配种,效益可能会更大。