Callaway T R, Anderson R C, Anderson T J, Poole T L, Bischoff K M, Kubena L F, Nisbet D J
Food and Feed Safety Research Unit, Southern Plains Agricultural Research Center, USDA, TX 77845, USA.
J Appl Microbiol. 2001 Sep;91(3):427-34. doi: 10.1046/j.1365-2672.2001.01396.x.
Chlorate kills Escherichia coli O157:H7 and may be an effective feed additive for use in food animals. This study was designed to determine if development of chlorate-resistant E. coli O157:H7 strains was likely.
Escherichia coli O157:H7 was chlorate-sensitive, but became chlorate-resistant in pure batch culture and in sterilized faecal fluid; it was killed in mixed culture and did not become resistant. Addition of chlorate to continuous pure cultures caused chlorate resistance, but chlorate addition to mixed continuous culture eliminated E. coli O157:H7 and no resistance occurred. Piglets challenged with E. coli O157:H7 were treated with chlorate; populations were reduced and colonies were always chlorate-sensitive.
Chlorate-resistant E. coli O157:H7 can be selected in pure, but not mixed culture, and results suggest that terminal chlorate feeding will not select for chlorate-resistance in vivo.
Chlorate can reduce food-borne pathogens prior to harvest, but development of resistance does not appear likely in vivo.
氯酸盐可杀死大肠杆菌O157:H7，可能是一种用于食用动物的有效饲料添加剂。本研究旨在确定是否可能出现耐氯酸盐的大肠杆菌O157:H7菌株。
大肠杆菌O157:H7对氯酸盐敏感，但在纯分批培养和无菌粪便液中会变得耐氯酸盐；在混合培养中会被杀死且不会产生抗性。向连续纯培养物中添加氯酸盐会导致耐氯酸盐，但向混合连续培养物中添加氯酸盐会消除大肠杆菌O157:H7且不会产生抗性。用氯酸盐处理感染大肠杆菌O157:H7的仔猪；菌量减少，菌落始终对氯酸盐敏感。
耐氯酸盐的大肠杆菌O157:H7可在纯培养而非混合培养中被筛选出来，结果表明在体内进行终末氯酸盐投喂不会筛选出耐氯酸盐菌株。
氯酸盐可在收获前减少食源性病原体，但在体内似乎不太可能产生抗性。
