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通过对扩增的16S rDNA进行限制性分析,从浅层热液喷口鉴定嗜热和海洋杆菌。

Identification of thermophilic and marine bacilli from shallow thermal vents by restriction analysis of their amplified 16S rDNA.

作者信息

Caccamo D, Maugeri T L, Gugliandolo C

机构信息

Dipartimento di Biologia Animale ed Ecologia Marina, Sez. Ecologia Microbica e Biotecnologie, University of Messina, Messina, Italy.

出版信息

J Appl Microbiol. 2001 Sep;91(3):520-4. doi: 10.1046/j.1365-2672.2001.01410.x.

Abstract

AIMS

In order to identify 73 thermophilic isolates from shallow, marine thermal vents of Eolian Islands, we compared their restriction patterns of amplified 16S rDNA with those of nine well described Bacillus species and eight Eolian Bacillus strains.

METHODS AND RESULTS

This study allowed to assign 57 (78%) isolates to different Bacillus species. Nineteen field strains were recognised as representatives of four described species, namely B. thermodenitrificans, "B. caldolyticus", B. vulcani and B. stearothermophilus. The profiles of 38 isolates matched instead, those of seven Eolian strains (B. thermodenitrificans strain A2, B. licheniformis strain B3-15, and five novel species, represented by Bacillus strain 1bw, Bacillus strain 4-1, Bacillus strain 5-2, Bacillus strain 10-1, Bacillus strain 1as). Among the 16 unidentified isolates, seven restriction patterns were recognised.

CONCLUSIONS

This study showed that restriction analysis of amplified 16S rDNA is useful for a rapid and reliable identification of strains belonging to described species as well as for recognition of new species.

SIGNIFICANCE AND IMPACT OF THE STUDY

This work revealed a high taxonomic diversity among the thermophilic bacilli isolated from Eolian Islands and a distinct distribution of the species within the Eolian hydrothermal vent system.

摘要

目的

为了鉴定从伊奥利亚群岛浅海热液喷口分离出的73株嗜热菌,我们将它们扩增的16S rDNA的限制性酶切图谱与9种已详细描述的芽孢杆菌属物种和8株伊奥利亚芽孢杆菌菌株的图谱进行了比较。

方法与结果

本研究将57株(78%)分离株归为不同的芽孢杆菌属物种。19株野外菌株被鉴定为4种已描述物种的代表,即嗜热脱氮芽孢杆菌、“解热芽孢杆菌”、火山芽孢杆菌和嗜热脂肪芽孢杆菌。相反,38株分离株的图谱与7株伊奥利亚菌株(嗜热脱氮芽孢杆菌A2菌株、地衣芽孢杆菌B3 - 15菌株,以及以芽孢杆菌1bw菌株、芽孢杆菌4 - 1菌株、芽孢杆菌5 - 2菌株、芽孢杆菌10 - 1菌株、芽孢杆菌1as菌株为代表的5个新物种)的图谱相匹配。在16株未鉴定的分离株中,识别出7种限制性酶切图谱。

结论

本研究表明,扩增的16S rDNA的限制性分析对于快速可靠地鉴定已描述物种的菌株以及识别新物种很有用。

研究的意义和影响

这项工作揭示了从伊奥利亚群岛分离出的嗜热芽孢杆菌之间存在高度的分类多样性,以及这些物种在伊奥利亚热液喷口系统中的独特分布。

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