Blanc M, Marilley L, Beffa T, Aragno M
Laboratoire de Microbiologie, Université de Neuchâtel, Switzerland.
Int J Syst Bacteriol. 1997 Oct;47(4):1246-8. doi: 10.1099/00207713-47-4-1246.
The restriction enzyme profiles of 16S ribosomal DNAs (rDNAs) amplified by PCR from thermophilic heterotrophic bacterial strains isolated from composts were compared with those of reference strains. This allowed us to assign all but 1 of 16 strains to four different Bacillus species (namely, Bacillus stearothermophilus, Bacillus pallidus, Bacillus thermoglucosidasius, and "Bacillus thermodenitrificans"). This study showed that PCR restriction analysis of 16S rDNA contributes to rapid and reliable identification of newly isolated strains belonging to recognized species.
将通过聚合酶链反应（PCR）从堆肥中分离出的嗜热异养细菌菌株扩增得到的16S核糖体DNA（rDNA）的限制性酶切图谱，与参考菌株的图谱进行了比较。这使我们能够将16个菌株中的15个菌株归为4个不同的芽孢杆菌属物种（即嗜热脂肪芽孢杆菌、苍白芽孢杆菌、嗜热葡萄糖苷芽孢杆菌和“嗜热脱硝芽孢杆菌”）。这项研究表明，16S rDNA的PCR限制性分析有助于快速、可靠地鉴定属于已知物种的新分离菌株。
