柑橘杂色黄化病的病原菌——桑氏木质部小菌的随机扩增多态性DNA图谱及抗生素敏感性
RAPD profile and antibiotic susceptibility of Xylella fastidiosa, causal agent of citrus variegated chlorosis.
作者信息
Lacava P T, Araújo W L, Maccheroni W, Azevedo J L
机构信息
Genetics Department, Escola Superior de Agricultura Luiz de Queiroz-USP, Piracicaba, São Paulo, Brazil.
出版信息
Lett Appl Microbiol. 2001 Oct;33(4):302-6. doi: 10.1046/j.1472-765x.2001.01000.x.
AIMS
The aim of this study was to evaluate the diversity of Xylella fastidiosa isolated from citrus trees affected by Citrus Variegated Chlorosis (CVC).
METHODS AND RESULTS
The antibiotic susceptibility by agar disc diffusion and minimum inhibitory concentration (MIC) methods was observed for all drug evaluated, except for penicillin-G. Genetic diversity by RAPD analysis revealed three major groups (citrus, coffee and grapevine), being the citrus group more similar with the coffee group than with the grapevine group.
CONCLUSIONS, SIGNIFICANCE AND IMPACT OF THE STUDY: This study highlights the possibility to use these antibiotics susceptibility as markers in the development of a cloning vector and penicillin-G could be used as a selective marker for the isolation of X. fastidiosa from citrus plants.
目的
本研究旨在评估从受柑橘杂色黄化病（CVC）影响的柑橘树上分离出的木质部难养菌的多样性。
方法与结果
除青霉素G外，对所有评估药物采用琼脂纸片扩散法和最低抑菌浓度（MIC）法观察抗生素敏感性。通过RAPD分析的遗传多样性揭示了三个主要群体（柑橘、咖啡和葡萄），柑橘群体与咖啡群体的相似性高于与葡萄群体的相似性。
研究的结论、意义和影响：本研究强调了将这些抗生素敏感性用作克隆载体开发中的标记的可能性，并且青霉素G可作为从柑橘植物中分离木质部难养菌的选择标记。
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