Fujimori M, Ikeda S, Shimizu Y, Okajima M, Asahara T
Second Department of Surgery, Hiroshima University Faculty of Medicine, Hiroshima, Japan 734-8551.
Cancer Res. 2001 Sep 15;61(18):6656-9.
The molecular basis of carcinogenesis in gastrointestinal carcinoid tumors is not well understood. To clarify the contribution of the Wnt/beta-catenin signaling to this type of carcinogenesis, we investigated 72 cases of gastrointestinal carcinoid tumor both immunohistochemically and by direct sequencing of beta-catenin. Accumulation of beta-catenin in the cytoplasm and/or nucleus was observed in 57 cases (79.2%). We also detected mutations in exon 3 of beta-catenin in 27 cases (37.5%) and one mutation in APC (1.4%). Our results suggest that alterations in the Wnt/beta-catenin signaling pathway may be involved in the development of gastrointestinal carcinoid tumors.
胃肠道类癌肿瘤发生癌变的分子基础尚未完全明确。为阐明Wnt/β-连环蛋白信号传导在此类癌变过程中的作用，我们采用免疫组织化学和β-连环蛋白直接测序的方法，对72例胃肠道类癌肿瘤进行了研究。在57例（79.2%）病例中观察到β-连环蛋白在细胞质和/或细胞核中积聚。我们还在27例（37.5%）病例中检测到β-连环蛋白第3外显子的突变，以及1例（1.4%）病例中检测到APC的一个突变。我们的结果表明，Wnt/β-连环蛋白信号通路的改变可能参与了胃肠道类癌肿瘤的发生发展。
