采用2-(4-羧基苯基)-5,6-二甲基苯并咪唑柱前衍生高效液相色谱法灵敏测定双酚A和烷基酚。

Sensitive determination of bisphenol A and alkylphenols by high performance liquid chromatography with pre-column derivatization with 2-(4-carboxyphenyl)-5,6-dimethylbenzimidazole.

作者信息

Katayama M, Sasaki T, Matsuda Y, Kaneko S, Iwamoto T, Tanaka M

机构信息

Department of Functional Bioanalysis, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.

出版信息

Biomed Chromatogr. 2001 Oct;15(6):403-7. doi: 10.1002/bmc.88.

DOI:10.1002/bmc.88

PMID:11559925

Abstract

A new and sensitive high-performance chromatographic method for the determination of bisphenol A and 8 alkylphenols with fluorescence detection is reported. Each phenol was derivatized by reaction with 2-(4-carboxyphenyl)-5,6-dimethylbenzimidazole at 40 degrees C for 60 min. The fluorescence derivatives were separated on a Wakosil 5C18 column (4.0 i.d. x 300 mm, 5 microm) with methanol:water (10:90) as mobile phase (detection wavelength: lambda(ex) 336 nm, lambda(em) 440 nm). The detection limits were in the range of 0.1-10.0 pg/mL in serum. The calibration graphs were linear to 1.0 microg/mL. The relative standard deviations were 7.2-8.9%, respectively. The proposed method was applied to the determination of bisphenol A in mother and infant rat serum.

摘要

报道了一种用于测定双酚A和8种烷基酚的新型灵敏的高效色谱方法，采用荧光检测。每种酚类通过与2-(4-羧基苯基)-5,6-二甲基苯并咪唑在40℃反应60分钟进行衍生化。荧光衍生物在Wakosil 5C18柱（内径4.0×300mm，5μm）上分离，以甲醇:水（10:90）作为流动相（检测波长：激发波长336nm，发射波长440nm）。血清中的检测限在0.1 - 10.0 pg/mL范围内。校准曲线在1.0μg/mL范围内呈线性。相对标准偏差分别为7.2 - 8.9%。该方法应用于母婴大鼠血清中双酚A的测定。